|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
|
|
|
Deletion
|
OCR30
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
ilvI
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
ilvI
Search strains by all related name:
ilvI b0077 ECK0079 JW0076 o604
|
General information (Go to Linear View: )
|
|
Gene Name
|
ilvI
|
|
Alternative name
|
b0077,ECK0079,JW0076,o604
|
|
Location, Length
|
85,630 - 87,354
(
+
)
;
1.85
min
;
1725
(bp) ,
574
(aa)
|
|
Product
|
acetolactate synthase III, large subunit
|
|
Operon Name
|
ilvIH
|
|
Note
|
acetolactate synthase III, valine sensitive, large subunit
|
|
Function
|
enzyme; Amino acid biosynthesis: Isoleucine, Valine
|
|
Gene Ontology
|
GO:0000287
;
magnesium ion binding ( ilvI )
GO:0003824
;
catalytic activity ( ilvI )
GO:0003984
;
acetolactate synthase activity ( ilvI )
GO:0008652
;
amino acid biosynthetic process ( ilvI )
GO:0009082
;
branched chain family amino acid biosynthetic process ( ilvI )
GO:0016740
;
transferase activity ( ilvI )
GO:0030976
;
thiamin pyrophosphate binding ( ilvI )
GO:0046872
;
metal ion binding ( ilvI )
GO:0050660
;
FAD binding ( ilvI )
|
|
PID
|
87081685
|
EC number
(KEGG Pathway)
|
2.2.1.6
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
ilvI
(
EG10500
)
|
|
Number of removed
|
0 aa cleaved
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
ILVI_ECOLI
(
P00893
;
P78045
)
|
|
- Protein name
|
Acetolactate synthase isozyme III large subunit
|
|
- Synonyms
|
EC 2.2.1.6, AHAS- III, Acetohydroxy-acid synthase III large subunit, ALS-III
|
|
- Gene name
|
Name=ilvI; OrderedLocusNames=b0077;
|
|
MMBR References
|
J Bacteriol. 1982;149(1):387-90.
Acetohydroxy acid synthase is a target for leucine containing peptide toxicity in Escherichia coli.
Gollop N, Tavori H, Barak Z.
(
7033214
)
|
J Bacteriol. 1985;163(1):186-98.
Unusual organization of the ilvIH promoter of Escherichia coli.
Haughn GW, Squires CH, De Felice M, Largo CT, Calvo JM.
(
3891724
)
|
FEMS Microbiol Lett. 1995;131(1):95-8.
Control of ilvIH transcription during amino acid downshift in stringent and relaxed strains of Escherichia coli.
Baccigalupi L, Marasco R, Ricca E, De Felice M, Sacco M.
(
7557316
)
|
J Bacteriol. 1990;172(8):4563-70.
The ilvIH operon of Escherichia coli is positively regulated.
Platko JV, Willins DA, Calvo JM.
(
2115869
)
|
J Bacteriol. 1965;89:654-60.
REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. II. IDENTIFICATION OF TWO OPERATOR GENES.
Ramakrishnan T, Adelberg EA.
(
14273640
)
|
J Bacteriol. 1981;147(3):797-804.
Physical characterization of the ilvHI operon of Escherichia coli K-12.
Squires CH, De Felice M, Wessler SR, Calvo JM.
(
6168634
)
|
Nucleic Acids Res. 1983;11:5299-5313
Molecular structure of ilvIH and its evolutionary relationship to ilvG in Escherichia coli K-12.
Squires, C. H., M. DeFelice, J. Devereux, and J. M. Calvo.
|
J Bacteriol. 1981;148(3):998-1001.
Valine-resistant Escherichia coli K-12 strains with mutations in the ilvB operon.
Sutton A, Newman T, Francis M, Freundlich M.
(
7031038
)
|
Curr. Top. Microbiol. Immunol. 1969;1:57-76.
Regulation of the biosynthesis of the branched-chain amino acids.
Umbarger, H. E.
|
Mol Gen Genet. 1977;156(3):239-49.
Valine-sensitive acetohydroxy acid synthases in Escherichia coli K-12: unique regulation modulated by multiple genetic sites.
Davis EJ, Blatt JM, Henderson EK, Whittaker JJ, Jackson JH.
(
340906
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MEMLSGAEMV VRSLIDQGVK QVFGYPGGAV LDIYDALHTV GGIDHVLVRH EQAAVHMADG LARATGEVGV
0071 VLVTSGPGAT NAITGIATAY MDSIPLVVLS GQVATSLIGY DAFQECDMVG ISRPVVKHSF LVKQTEDIPQ
0141 VLKKAFWLAA SGRPGPVVVD LPKDILNPAN KLPYVWPESV SMRSYNPTTT GHKGQIKRAL QTLVAAKKPV
0211 VYVGGGAITA GCHQQLKETV EALNLPVVCS LMGLGAFPAT HRQALGMLGM HGTYEANMTM HNADVIFAVG
0281 VRFDDRTTNN LAKYCPNATV LHIDIDPTSI SKTVTADIPI VGDARQVLEQ MLELLSQESA HQPLDEIRDW
0351 WQQIEQWRAR QCLKYDTHSE KIKPQAVIET LWRLTKGDAY VTSDVGQHQM FAALYYPFDK PRRWINSGGL
0421 GTMGFGLPAA LGVKMALPEE TVVCVTGDGS IQMNIQELST ALQYELPVLV VNLNNRYLGM VKQWQDMIYS
0491 GRHSQSYMQS LPDFVRLAEA YGHVGIQISH PHELESKLSE ALEQVRNNRL VFVDVTVDGS EHVYPMQIRG
0561 GGMDEMWLSK TERT
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 TTATTACCCC GTGTTTATGT CTCTGGCTGC
-070 CAATTGCTTA AGCAAGATCG GACGGTTAAT GTGTTTTACA CATTTTTTCC GTCAAACAGT GAGGCAGGCC
0001 atggagatgt tgtctggagc cgagatggtc gtccgatcgc ttatcgatca gggcgttaaa caagtattcg
0071 gttatcccgg aggcgcagtc cttgatattt atgatgcatt gcataccgtg ggtggtattg atcatgtatt
0141 agttcgtcat gagcaggcgg cggtgcatat ggccgatggc ctggcgcgcg cgaccgggga agtcggcgtc
0211 gtgctggtaa cgtcgggtcc aggggcgacc aatgcgatta ctggcatcgc caccgcttat atggattcca
0281 ttccattagt tgtcctttcc gggcaggtag cgacctcgtt gataggttac gatgcctttc aggagtgcga
0351 catggtgggg atttcgcgac cggtggttaa acacagtttt ctggttaagc aaacggaaga cattccgcag
0421 gtgctgaaaa aggctttctg gctggcggca agtggtcgcc caggaccagt agtcgttgat ttaccgaaag
0491 atattcttaa tccggcgaac aaattaccct atgtctggcc ggagtcggtc agtatgcgtt cttacaatcc
0561 cactactacc ggacataaag ggcaaattaa gcgtgctctg caaacgctgg tagcggcaaa aaaaccggtt
0631 gtctacgtag gcggtggggc aatcacggcg ggctgccatc agcagttgaa agaaacggtg gaggcgttga
0701 atctgcccgt tgtttgctca ttgatggggc tgggggcgtt tccggcaacg catcgtcagg cactgggcat
0771 gctgggaatg cacggtacct acgaagccaa tatgacgatg cataacgcgg atgtgatttt cgccgtcggg
0841 gtacgatttg atgaccgaac gacgaacaat ctggcaaagt actgcccaaa tgccactgtt ctgcatatcg
0911 atattgatcc tacttccatt tctaaaaccg tgactgcgga tatcccgatt gtgggggatg ctcgccaggt
0981 cctcgaacaa atgcttgaac tcttgtcgca agaatccgcc catcaaccac tggatgagat ccgcgactgg
1051 tggcagcaaa ttgaacagtg gcgcgctcgt cagtgcctga aatatgacac tcacagtgaa aagattaaac
1121 cgcaggcggt gatcgagact ctttggcggt tgacgaaggg agacgcttac gtgacgtccg atgtcgggca
1191 gcaccagatg tttgctgcac tttattatcc attcgacaaa ccgcgtcgct ggatcaattc cggtggcctc
1261 ggcacgatgg gttttggttt acctgcggca ctgggcgtca aaatggcgtt gccagaagaa accgtggttt
1331 gcgtcactgg cgacggcagt attcagatga acatccagga actgtctacc gcgttgcaat acgagttgcc
1401 cgtactggtg gtgaatctca ataaccgcta tctggggatg gtgaagcagt ggcaggacat gatctattcc
1471 ggccgtcatt cacaatctta tatgcaatcg ctacccgatt tcgtccgtct ggcggaagcc tatgggcatg
1541 tcgggatcca gatttctcat ccgcatgagc tggaaagcaa acttagcgag gcgctggaac aggtgcgcaa
1611 taatcgcctg gtgtttgttg atgttaccgt cgatggcagc gagcacgtct acccgatgca gattcgcggg
1681 ggcggaatgg atgaaatgtg gttaagcaaa acggagagaa cctgaTTATG CGCCGGATAT TATCAGTCTT
1751 ACTCGAAAAT GAATCAGGCG CGTTATCCCG CGTGATTGGC CTTTTTTCCC AGCGTGGCTA CAACATTGAA
1821 AGCCT
|
