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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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Annu Rev Biochem. 1995;64:171-200.
DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine.
Kelman Z, O'Donnell M.
(
7574479
)
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Deletion
|
OCR46-11
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
holA
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Related strains
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Strains Search
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Search strains by gene name:
holA
Search strains by all related name:
holA b0640 ECK0633 JW0635
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General information (Go to Linear View: )
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Gene Name
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holA
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Alternative name
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b0640,ECK0633,JW0635
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Location, Length
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669,797 - 670,828
(
-
)
;
14.44
min
;
1032
(bp) ,
343
(aa)
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Product
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DNA polymerase III, delta subunit
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Operon Name
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leuS-lptE-holA-nadD-cobC
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Note
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GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006261 - DNA-dependent DNA replication
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Function
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enzyme; DNA - replication, repair,restriction/modification
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Gene Ontology
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GO:0003677
;
DNA binding ( holA )
GO:0003887
;
DNA-directed DNA polymerase activity ( holA )
GO:0003891
;
delta DNA polymerase activity ( holA )
GO:0005515
;
protein binding ( holA )
GO:0005634
;
nucleus ( holA )
GO:0006260
;
DNA replication ( holA )
GO:0009360
;
DNA polymerase III complex ( holA )
GO:0016740
;
transferase activity ( holA )
GO:0016779
;
nucleotidyltransferase activity ( holA )
GO:0043625
;
delta DNA polymerase complex ( holA )
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PID
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1786859
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EC number
(KEGG Pathway)
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2.7.7.7
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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holA
(
EG11412
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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HOLA_ECOLI
(
P28630
)
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- Protein name
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DNA polymerase III, delta subunit
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- Synonyms
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EC 2.7.7.7
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- Gene name
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Name=holA; OrderedLocusNames=b0640;
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MMBR References
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J Biol Chem. 1993;268:11758-11765
DNA polymerase III accessory proteins. I. holA and holB encoding delta and delta'.
Dong, Z., R. Onrust, M. Skangalis, and M. E. O'Donnell.
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Annu Rev Biochem. 1995;64:171-200.
DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine.
Kelman Z, O'Donnell M.
(
7574479
)
|
J Biol Chem. 1993;268:11766-11772
DNA polymerase III accessory proteins. II. Characterization of delta and delta'.
Onrust, R., and M. O'Donnell.
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J Bacteriol. 1992;174:7013-7025
Molecular cloning, sequencing, and overexpression of the structural gene encoding the subunit of Escherichia coli DNA polymerase III holoenzyme.
Carter, J. R., M. A. Franden, R. Aebersold, and C. S. McHenry.
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| Sequences |
Amino acid
FASTA format
|
0001 MIRLYPEQLR AQLNEGLRAA YLLLGNDPLL LQESQDAVRQ VAAAQGFEEH HTFSIDPNTD WNAIFSLCQA
0071 MSLFASRQTL LLLLPENGPN AAINEQLLTL TGLLHDDLLL IVRGNKLSKA QENAAWFTAL ANRSVQVTCQ
0141 TPEQAQLPRW VAARAKQLNL ELDDAANQVL CYCYEGNLLA LAQALERLSL LWPDGKLTLP RVEQAVNDAA
0211 HFTPFHWVDA LLMGKSKRAL HILQQLRLEG SEPVILLRTL QRELLLLVNL KRQSAHTPLR ALFDKHRVWQ
0281 NRRGMMGEAL NRLSQTQLRQ AVQLLTRTEL TLKQDYGQSV WAELEGLSLL LCHKPLADVF IDG
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 gcatccgtgc tgcggatatt cgttccgacg
-070 aagaacagac gtcgaccaca acggatactc cggcaacgcc tgcacgcgtc tccaccacgc tgggtaactg
0001 atgattcggt tgtacccgga acaactccgc gcgcagctca atgaagggct gcgcgcggcg tatcttttac
0071 ttggtaacga tcctctgtta ttgcaggaaa gccaggacgc tgttcgtcag gtagctgcgg cacaaggatt
0141 cgaagaacac cacacttttt ccattgatcc caacactgac tggaatgcga tcttttcgtt atgccaggct
0211 atgagtctgt ttgccagtcg acaaacgcta ttgctgttgt taccagaaaa cggaccgaat gcggcgatca
0281 atgagcaact tctcacactc accggacttc tgcatgacga cctgctgttg atcgtccgcg gtaataaatt
0351 aagcaaagcg caagaaaatg ccgcctggtt tactgcgctt gcgaatcgca gcgtgcaggt gacctgtcag
0421 acaccggagc aggctcagct tccccgctgg gttgctgcgc gcgcaaaaca gctcaactta gaactggatg
0491 acgcggcaaa tcaggtgctc tgctactgtt atgaaggtaa cctgctggcg ctggctcagg cactggagcg
0561 tttatcgctg ctctggccag acggcaaatt gacattaccg cgcgttgaac aggcggtgaa tgatgccgcg
0631 catttcaccc cttttcattg ggttgatgct ttgttgatgg gaaaaagtaa gcgcgcattg catattcttc
0701 agcaactgcg tctggaaggc agcgaaccgg ttattttgtt gcgcacatta caacgtgaac tgttgttact
0771 ggttaacctg aaacgccagt ctgcccatac gccactgcgt gcgttgtttg ataagcatcg ggtatggcag
0841 aaccgccggg gcatgatggg cgaggcgtta aatcgcttaa gtcagacgca gttacgtcag gccgtgcaac
0911 tcctgacacg aacggaactc accctcaaac aagattacgg tcagtcagtg tgggcagagc tggaagggtt
0981 atctcttctg ttgtgccata aacccctggc ggacgtattt atcgacggtt gatatgaaat ctttacaggc
1051 tctgtttggc ggcacctttg atccggtgca ctatggtcat ctaaaacccg tggaaacgct ggcgaatttg
1121 attggtctga cg
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