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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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J Biol Chem. 1998;273(24):15157-61.
Disruption of Escherichia coli hepA, an RNA polymerase-associated protein, causes UV sensitivity.
Muzzin O, Campbell EA, Xia L, Severinova E, Darst SA, Severinov K.
(
9614128
)
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Deletion
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OCR29
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
rapA
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Related strains
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Strains Search
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Search strains by gene name:
rapA
Search strains by all related name:
rapA b0059 ECK0060 hepA JW0058 yabA
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General information (Go to Linear View: )
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Gene Name
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rapA
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Alternative name
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b0059,ECK0060,hepA,JW0058,yabA
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Location, Length
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60,358 - 63,264
(
-
)
;
1.30
min
;
2907
(bp) ,
968
(aa)
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Product
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RNA polymerase-associated helicase protein (ATPase and RNA polymerase recycling factor)
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Operon Name
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hepA-rluA
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Note
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probable ATP-dependent RNA helicase; GO_process: GO:0009451 - RNA modification
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Function
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putative enzyme; RNA synthesis, modification, DNA transcription
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Gene Ontology
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GO:0000166
;
nucleotide binding ( rapA )
GO:0003676
;
nucleic acid binding ( rapA )
GO:0003677
;
DNA binding ( rapA )
GO:0004386
;
helicase activity ( rapA )
GO:0005524
;
ATP binding ( rapA )
GO:0006350
;
transcription ( rapA )
GO:0006355
;
regulation of transcription, DNA-dependent ( rapA )
GO:0016787
;
hydrolase activity ( rapA )
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PID
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1786245
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EC number
(KEGG Pathway)
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3.6.1.-
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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hepA
(
EG11083
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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RAPA_ECOLI
(
P60240
;
P23852; P75633
)
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- Protein name
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RNA polymerase associated protein rapA
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- Synonyms
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EC 3.6.1.-, ATP-dependent helicase hepA
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- Gene name
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Name=rapA; Synonyms=hepA; OrderedLocusNames=b0059;
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MMBR References
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Nucleic Acids Res. 1995;23:2715-2723
Evolution of the SNF2 family of proteins: subfamilies with distinct sequences and functions.
Eisen, J. A., K. S. Sweder, and P. C. Hanawalt.
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J Bacteriol. 1992;174:3377-3385
Isolation of DNA damage-inducible promoters in Escherichia coli: regulation of polB (dinA), dinG, and dinH by LexA repressor.
Lewis, L. K., M. E. Jenkins, and D. W. Mount.
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J Biol Chem. 1998;273:7018-7023
RapA, a novel RNA polymerase-associated protein, is a bacterial homolog of SWI2/SNF2.
Sukhodolets, M. V., and D. J. Jin.
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Nucleic Acids Res. 1993;21(3):751-2.
An expanding family of helicases within the 'DEAD/H' superfamily.
Bork P, Koonin EV.
(
8382805
)
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| Sequences |
Amino acid
FASTA format
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0001 MPFTLGQRWI SDTESELGLG TVVAVDARTV TLLFPSTGEN RLYARSDSPV TRVMFNPGDT ITSHDGWQMQ
0071 VEEVKEENGL LTYIGTRLDT EESGVALREV FLDSKLVFSK PQDRLFAGQI DRMDRFALRY RARKYSSEQF
0141 RMPYSGLRGQ RTSLIPHQLN IAHDVGRRHA PRVLLADEVG LGKTIEAGMI LHQQLLSGAA ERVLIIVPET
0211 LQHQWLVEML RRFNLRFALF DDERYAEAQH DAYNPFDTEQ LVICSLDFAR RSKQRLEHLC EAEWDLLVVD
0281 EAHHLVWSED APSREYQAIE QLAEHVPGVL LLTATPEQLG MESHFARLRL LDPNRFHDFA QFVEEQKNYR
0351 PVADAVAMLL AGNKLSNDEL NMLGEMIGEQ DIEPLLQAAN SDSEDAQSAR QELVSMLMDR HGTSRVLFRN
0421 TRNGVKGFPK RELHTIKLPL PTQYQTAIKV SGIMGARKSA EDRARDMLYP ERIYQEFEGD NATWWNFDPR
0491 VEWLMGYLTS HRSQKVLVIC AKAATALQLE QVLREREGIR AAVFHEGMSI IERDRAAAWF AEEDTGAQVL
0561 LCSEIGSEGR NFQFASHMVM FDLPFNPDLL EQRIGRLDRI GQAHDIQIHV PYLEKTAQSV LVRWYHEGLD
0631 AFEHTCPTGR TIYDSVYNDL INYLASPDQT EGFDDLIKNC REQHEALKAQ LEQGRDRLLE IHSNGGEKAQ
0701 ALAESIEEQD DDTNLIAFAM NLFDIIGINQ DDRGDNMIVL TPSDHMLVPD FPGLSEDGIT ITFDREVALA
0771 REDAQFITWE HPLIRNGLDL ILSGDTGSST ISLLKNKALP VGTLLVELIY VVEAQAPKQL QLNRFLPPTP
0841 VRMLLDKNGN NLAAQVEFET FNRQLNAVNR HTGSKLVNAV QQDVHAILQL GEAQIEKSAR ALIDAARNEA
0911 DEKLSAELSR LEALRAVNPN IRDDELTAIE SNRQQVMESL DQAGWRLDAL RLIVVTHQ
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 tagcgccctt tccattcctg gacctgaata
-070 acaccactac ctcataagca cggtagcggg tggttattgc ctgcaattaa agatatagag ccgaacacat
0001 atgcctttta cacttggtca acgctggatc agcgatacag aaagcgaatt gggacttgga accgttgtcg
0071 cggtggatgc gcgaactgtc actttacttt tcccatctac tggtgaaaac cgtctgtacg cacgcagtga
0141 ttcccccgtg acccgcgtga tgttcaaccc tggtgatacc attaccagcc atgacggctg gcagatgcaa
0211 gtcgaagaag taaaagaaga aaatggcttg ctgacctata tcggtactcg cctggatact gaagagtccg
0281 gcgtagccct gcgtgaagtt ttccttgata gcaaactggt gttcagcaaa ccgcaggacc gtctgtttgc
0351 cgggcagatt gaccgtatgg accgctttgc gctgcgttat cgcgcgcgta aatattccag cgaacagttc
0421 cgtatgccgt acagcggcct gcgcggtcag cgtaccagcc tgatcccgca tcagctcaac atcgctcatg
0491 atgttggtcg ccgccacgcg ccgcgcgtcc tgctggctga cgaagtgggt ttagggaaaa ccattgaagc
0561 cgggatgatc ctgcatcagc aactgctctc tggcgctgct gaacgtgtgc taattatcgt cccggaaacc
0631 ttacagcatc agtggctggt agaaatgctg cgccgtttca acctgcgctt tgcgctattt gatgatgagc
0701 gttatgccga agctcagcac gatgcttaca acccgtttga caccgaacag ctggtgattt gctcgctgga
0771 ttttgcccgt cgtagcaaac agcgcctgga acatctctgt gaagccgaat gggacctgct ggtggtcgat
0841 gaagcgcatc acctggtgtg gagcgaagat gcgccaagcc gtgaatatca ggccattgaa caactggcag
0911 agcacgtgcc gggcgttctg ctgctgaccg cgaccccgga acagctgggg atggaaagcc acttcgcccg
0981 tctgcgtctg ctggacccga accgtttcca cgattttgcg cagttcgttg aagagcagaa aaattatcgt
1051 ccggttgcgg acgccgttgc catgctgctg gcaggtaaca aactgagcaa tgacgaactg aacatgctcg
1121 gcgagatgat cggcgagcag gatatcgagc cgctgttgca ggcagcaaac agcgacagcg aagatgccca
1191 gagcgcccgt caggagctgg tttcgatgct gatggatcgc cacggcacca gccgcgtgct gttccgtaac
1261 acgcgtaacg gtgtgaaagg attcccgaaa cgcgagctgc acaccattaa gctgccgcta ccgacgcagt
1331 atcagacggc tattaaagtc tccggcatta tgggcgcacg taaaagtgcg gaagatcgtg ctcgcgatat
1401 gctctacccg gagcgtattt atcaggaatt tgaaggtgat aacgccacct ggtggaactt cgatccgcgc
1471 gttgagtggc tgatgggcta cctgaccagc catcgctctc agaaagtgct ggtgatctgc gccaaagctg
1541 ccactgcgct gcaactggag caggtactgc gcgaacgtga aggtattcgc gctgcggtgt tccacgaagg
1611 tatgtcgatt atcgaacgtg accgcgctgc cgcctggttt gccgaagaag acaccggcgc acaggtactg
1681 ctgtgctcag aaatcggttc tgaaggacgt aacttccagt tcgccagcca catggtgatg tttgacctgc
1751 cattcaaccc ggatctactg gagcagcgta ttggtcgtct ggatcgtatc ggccaggcgc acgatattca
1821 gatccatgtg ccttatctgg agaaaaccgc tcagtcggtg ctggtgcgct ggtatcacga aggtctggat
1891 gcatttgagc acacctgccc gaccggacgc actatttacg atagcgtata caacgatctg attaactatc
1961 tggcttcacc ggatcaaacc gaaggctttg acgatctgat caaaaactgc cgcgagcaac atgaagcgct
2031 gaaagcacag ctggaacagg gtcgtgaccg cctgctggaa atccactcca acggtggcga aaaagcccag
2101 gcactggcag aaagcattga agagcaggat gacgatacca acctgatcgc cttcgccatg aacctgttcg
2171 atattatcgg tatcaatcag gacgatcgcg gcgacaacat gatcgtgctg acgccgtccg atcatatgct
2241 ggtgccggac ttccctggcc tgtcggaaga tggcatcacc atcacctttg atcgtgaagt ggcgctggcg
2311 cgtgaagatg cacagtttat tacctgggag catccgctga tccgcaacgg tctggatctg atcctttctg
2381 gcgataccgg tagcagcacg atttcactgt taaaaaacaa agcgttgccg gtaggtacgc tgttggtgga
2451 actgatttat gtggttgaag cccaggctcc gaagcagttg cagctcaacc gcttcctgcc accgacgccg
2521 gtacgtatgc tgctggataa aaacggcaac aacctggcgg cgcaggtaga gtttgaaacc tttaaccgcc
2591 agcttaacgc ggttaaccgt cacaccggca gcaaactggt taacgccgtg cagcaggatg ttcacgctat
2661 ccttcaactg ggtgaagcgc agatcgagaa atctgcccgt gcattgattg atgcagcgcg taacgaagcc
2731 gacgaaaaac tgtctgccga gctgtctcgt ctggaagctc tgcgtgcagt gaacccgaac attcgtgacg
2801 acgaactgac cgccattgag agcaaccgtc agcaggtaat ggaaagcctg gatcaggcag gttggcgtct
2871 ggatgccctg cgtttgatcg ttgtaacgca tcagtaacgg agccgaaaat ggggatggaa aactacaatc
2941 caccgcagga accctggttg gttatcctgt atcaggatga ccatattatg gtggtcaaca agccgag
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