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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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J Gen Microbiol. 1979;113(1):155-64.
Isolation of haemin-requiring mutants of Escherichia coli K12.
McConville ML, Charles HP.
(
387909
)
|
|
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Deletion
|
OCR38
(D)
|
|
Related gene (W3110 PEC)
|
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Gene Search
|
Search MG1655 PEC by gene name:
hemB
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Related strains
|
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Strains Search
|
Search strains by gene name:
hemB
Search strains by all related name:
hemB b0369 ECK0366 JW0361 ncf
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General information (Go to Linear View: )
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Gene Name
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hemB
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Alternative name
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b0369,ECK0366,JW0361,ncf
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Location, Length
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387,977 - 388,951
(
-
)
;
8.36
min
;
975
(bp) ,
324
(aa)
|
|
Product
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5-aminolevulinate dehydratase (porphobilinogen synthase)
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Operon Name
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hemB
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Note
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5-aminolevulinate dehydratase = porphobilinogen synthase
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Function
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enzyme; Biosynthesis of cofactors, carriers: Heme, porphyrin
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Gene Ontology
|
GO:0000287
;
magnesium ion binding ( hemB )
GO:0003824
;
catalytic activity ( hemB )
GO:0004655
;
porphobilinogen synthase activity ( hemB )
GO:0006779
;
porphyrin biosynthetic process ( hemB )
GO:0008152
;
metabolic process ( hemB )
GO:0008270
;
zinc ion binding ( hemB )
GO:0016829
;
lyase activity ( hemB )
GO:0033014
;
tetrapyrrole biosynthetic process ( hemB )
GO:0046872
;
metal ion binding ( hemB )
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PID
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87081728
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EC number
(KEGG Pathway)
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4.2.1.24
|
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
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EcoGene
|
hemB
(
EG10428
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
|
HEM2_ECOLI
(
P15002
;
P78247
)
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- Protein name
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Delta-aminolevulinic acid dehydratase
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- Synonyms
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EC 4.2.1.24, Porphobilinogen synthase, ALAD, ALADH
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- Gene name
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Name=hemB; Synonyms=ncf; OrderedLocusNames=b0369, SF0249, S0270;
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MMBR References
|
Mol Gen Genet. 1988;214(3):503-8.
Nucleotide sequence of the hemB gene of Escherichia coli K12.
Echelard Y, Dymetryszyn J, Drolet M, Sasarman A.
(
2464127
)
|
J Bacteriol. 1992;174:2072-2077
Oxygen sensitivity of an Escherichia coli mutant.
Adler, H. I., R. Mural, and B. Suttle.
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Microbiol Immunol. 1991;35(4):289-301.
Genetic and molecular analysis of spontaneous respiratory deficient (res-) mutants of Escherichia coli K-12.
Lewis LA, Li KB, Gousse A, Pereira F, Pacheco N, Pierre S, Kodaman P, Lawson S.
(
1943842
)
|
Gene. 1989;75(1):177-84.
The structure of the Escherichia coli hemB gene.
Li JM, Russell CS, Cosloy SD.
(
2656410
)
|
J Bacteriol. 1988;170(2):1021-5.
Cloning of the Escherichia coli K-12 hemB gene.
Li JM, Umanoff H, Proenca R, Russell CS, Cosloy SD.
(
3276659
)
|
J Gen Microbiol. 1979;113(1):155-64.
Isolation of haemin-requiring mutants of Escherichia coli K12.
McConville ML, Charles HP.
(
387909
)
|
J Gen Microbiol. 1979;111(1):193-200.
Mutants of Escherichia coli K12 accumulating porphobilinogen: a new locus, hemC.
McConville ML, Charles HP.
(
379277
)
|
J Bacteriol. 1991;173(1):94-100.
delta-Aminolevulinic acid dehydratase deficiency can cause delta-aminolevulinate auxotrophy in Escherichia coli.
O'Neill GP, Thorbjarnardottir S, Michelsen U, Palsson S, Soll D, Eggertsson G.
(
1987138
)
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| Sequences |
Amino acid
FASTA format
|
0001 MTDLIQRPRR LRKSPALRAM FEETTLSLND LVLPIFVEEE IDDYKAVEAM PGVMRIPEKH LAREIERIAN
0071 AGIRSVMTFG ISHHTDETGS DAWREDGLVA RMSRICKQTV PEMIVMSDTC FCEYTSHGHC GVLCEHGVDN
0141 DATLENLGKQ AVVAAAAGAD FIAPSAAMDG QVQAIRQALD AAGFKDTAIM SYSTKFASSF YGPFREAAGS
0211 ALKGDRKSYQ MNPMNRREAI RESLLDEAQG ADCLMVKPAG AYLDIVRELR ERTELPIGAY QVSGEYAMIK
0281 FAALAGAIDE EKVVLESLGS IKRAGADLIF SYFALDLAEK KILR
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 tccattctct tcttgtcaac atcgcgacaa
-070 ctttcgtaaa acatccctac cctgcttcag gtatactatg cccctcgatt ccacaaacat caggcagacc
0001 atgacagact taatccaacg ccctcgtcgc ctgcgcaaat ctcctgcgct gcgcgctatg tttgaagaga
0071 caacacttag ccttaacgac ctggtgttgc cgatctttgt tgaagaagaa attgacgact acaaagccgt
0141 tgaagccatg ccaggcgtga tgcgcattcc agagaaacat ctggcacgcg aaattgaacg catcgccaac
0211 gccggtattc gttccgtgat gacttttggc atctctcacc ataccgatga aaccggcagc gatgcctggc
0281 gggaagatgg actggtggcg cgtatgtcgc gcatctgcaa gcagaccgtg ccagaaatga tcgttatgtc
0351 agacacctgc ttctgtgaat acacttctca cggtcactgc ggtgtgctgt gcgagcatgg cgtcgacaac
0421 gacgcgactc tggaaaattt aggcaagcaa gccgtggttg cagctgctgc aggtgcagac ttcatcgccc
0491 cttccgccgc gatggacggc caggtacagg cgattcgtca ggcgctggac gctgcgggat ttaaagatac
0561 ggcgattatg tcgtattcga ccaagttcgc ctcctccttt tatggcccgt tccgtgaagc tgccggaagc
0631 gcattaaaag gcgaccgcaa aagctatcag atgaacccaa tgaaccgtcg tgaggcgatt cgtgaatcac
0701 tgctggatga agcccagggc gcagactgcc tgatggttaa acctgctgga gcgtacctcg acatcgtgcg
0771 tgagctgcgt gaacgtactg aattgccgat tggcgcgtat caggtgagcg gtgagtatgc gatgattaag
0841 ttcgccgcgc tggcgggtgc tatagatgaa gagaaagtcg tgctcgaaag cttaggttcg attaagcgtg
0911 cgggtgcgga tctgattttc agctactttg cgctggattt ggctgagaag aagattctgc gttaattttg
0981 tttcctctca gaaaggggga gttaacctgt tgaaagtacg gtgaacgccc cctcacccta accctctccc
1051 caagggggcg aggggaccgt ccact
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