|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Proc Natl Acad Sci U S A. 1990;87(1):360-3.
Mutations in the bglY gene increase the frequency of spontaneous deletions in Escherichia coli K-12.
Lejeune P, Danchin A.
(
2404280
)
|
|
|
Deletion
|
15
(LD)
,
DE(pgl-grxA)502
,
DE(nadA-galE)35
,
15+dps
(LD)
,
OCR48,49-4
(D)
,
OCR48,50-8 (LD 15D::Cm)
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
galK
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
galK
Search strains by all related name:
galK b0757 ECK0746 galA JW0740
|
General information (Go to Linear View: )
|
|
Gene Name
|
galK
|
|
Alternative name
|
b0757,ECK0746,galA,JW0740
|
|
Location, Length
|
788,054 - 789,202
(
-
)
;
16.99
min
;
1149
(bp) ,
382
(aa)
|
|
Product
|
galactokinase
|
|
Operon Name
|
galETKM
|
|
Note
|
GO_component: GO:0005737 - cytoplasm; GO_function: GO:0042280 - cell surface antigen activity, host-interacting; GO_process: GO:0006012 - galactose metabolic process; GO_process: GO:0009242 - colanic acid biosynthetic process; GO_process: GO:0016052 - carbohydrate catabolic process; GO_process: GO:0009269 - response to desiccation
|
|
Function
|
enzyme; Degradation of small molecules: Carbon compounds
|
|
Gene Ontology
|
GO:0000166
;
nucleotide binding ( galK )
GO:0004335
;
galactokinase activity ( galK )
GO:0005524
;
ATP binding ( galK )
GO:0005737
;
cytoplasm ( galK )
GO:0005975
;
carbohydrate metabolic process ( galK )
GO:0006012
;
galactose metabolic process ( galK )
GO:0008152
;
metabolic process ( galK )
GO:0016301
;
kinase activity ( galK )
GO:0016310
;
phosphorylation ( galK )
GO:0016740
;
transferase activity ( galK )
GO:0016773
;
phosphotransferase activity, alcohol group as acceptor ( galK )
GO:0046835
;
carbohydrate phosphorylation ( galK )
|
|
PID
|
1786972
|
EC number
(KEGG Pathway)
|
2.7.1.6
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
galK
(
EG10363
)
|
|
Number of removed
|
1 aa cleaved
|
|
SWISS-PROT
(
Show details
[
1
more]
)
|
Entry name(Acc.no)
|
GAL1_ECOLI
(
P06976
)
|
|
- Protein name
|
Galactokinase
|
|
- Synonyms
|
EC 2.7.1.6, Galactose kinase
|
|
- Gene name
|
Name=galK; Synonyms=galA; OrderedLocusNames=b0757, z0927, ECs0785;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
galK
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Crystal Structure Of Galactokinase From Pyrococcus Horikoshi
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Galactokinase (Galactose kinase)
|
|
PSI-BLAST
|
Galactokinase
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
galactokinase [Escherichia coli O157:H7 str. Sakai]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
AMIDATION
PHOSPHOPANTETHEINE
GALACTOKINASE
GHMP_KINASES_ATP
|
|
MMBR References
|
J Biol Chem. 1974;249:143-148
Triornithine-resistant strains of Escherichia coli. Isolation, definition, and genetic studies.
Barak, Z., and C. Gilvarg.
|
Symp. Soc. Gen. Microbiol. 1960;10:115-131.
Genetic and functional aspects of galactose metabolism in Escherichia coli K-12.
Lederberg, E. M.
|
Mol Gen Genet. 1968;102(1):79-88.
Negative control of the galactose operon in E. coli.
Saedler H, Gullon A, Fiethen L, Starlinger P.
(
4883532
)
|
Proc Natl Acad Sci U S A. 1963;50:463-9.
INTERFERENCE WITH GLYCEROKINASE INDUCTION IN MUTANTS OF E. COLI ACCUMULATING GAL-1-P.
Sundararajan TA.
(
14067091
)
|
Mol Microbiol. 1993;10(2):245-51.
The galactose regulon of Escherichia coli.
Weickert MJ, Adhya S.
(
7934815
)
|
Proc Natl Acad Sci U S A. 1959;45:1786-1791.
Hereditary defects in galactose metabolism in Escherichia coli mutants. II. Galactose-induced sensitivity.
Yarmolinsky, M., H. Wiesmeyer, H. M. Kalckar, and E. Jordan.
|
GenBank submission X02306. 1995
Debouck, C.
|
Nucleic Acids Res. 1985;13:1841-1853
Structure of the galactokinase gene of Escherichia coli, the last (?) gene of the gal operon.
Debouck, C., A. Riccio, D. Schumperli, K. McKenney, J. Jeffers, C. Hughes, M. Rosenberg, M. Heusterspreute, and J. Davison.
|
| Sequences |
Amino acid
FASTA format
|
0001 MSLKEKTQSL FANAFGYPAT HTIQAPGRVN LIGEHTDYND GFVLPCAIDY QTVISCAPRD DRKVRVMAAD
0071 YENQLDEFSL DAPIVAHENY QWANYVRGVV KHLQLRNNSF GGVDMVISGN VPQGAGLSSS ASLEVAVGTV
0141 LQQLYHLPLD GAQIALNGQE AENQFVGCNC GIMDQLISAL GKKDHALLID CRSLGTKAVS MPKGVAVVII
0211 NSNFKRTLVG SEYNTRREQC ETGARFFQQP ALRDVTIEEF NAVAHELDPI VAKRVRHILT ENARTVEAAS
0281 ALEQGDLKRM GELMAESHAS MRDDFEITVP QIDTLVEIVK AVIGDKGGVR MTGGGFGGCI VALIPEELVP
0351 AVQQAVAEQY EAKTGIKETF YVCKPSQGAG QC
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 aatgctggca gagacccagc gagacctgac
-070 cgcagaacag gcagcagagc gtttgcgcgc agtcagcgat atccattttc gcgaatccgg agtgtaagaa
0001 atgagtctga aagaaaaaac acaatctctg tttgccaacg catttggcta ccctgccact cacaccattc
0071 aggcgcctgg ccgcgtgaat ttgattggtg aacacaccga ctacaacgac ggtttcgttc tgccctgcgc
0141 gattgattat caaaccgtga tcagttgtgc accacgcgat gaccgtaaag ttcgcgtgat ggcagccgat
0211 tatgaaaatc agctcgacga gttttccctc gatgcgccca ttgtcgcaca tgaaaactat caatgggcta
0281 actacgttcg tggcgtggtg aaacatctgc aactgcgtaa caacagcttc ggcggcgtgg acatggtgat
0351 cagcggcaat gtgccgcagg gtgccgggtt aagttcttcc gcttcactgg aagtcgcggt cggaaccgta
0421 ttgcagcagc tttatcatct gccgctggac ggcgcacaaa tcgcgcttaa cggtcaggaa gcagaaaacc
0491 agtttgtagg ctgtaactgc gggatcatgg atcagctaat ttccgcgctc ggcaagaaag atcatgcctt
0561 gctgatcgat tgccgctcac tggggaccaa agcagtttcc atgcccaaag gtgtggctgt cgtcatcatc
0631 aacagtaact tcaaacgtac cctggttggc agcgaataca acacccgtcg tgaacagtgc gaaaccggtg
0701 cgcgtttctt ccagcagcca gccctgcgtg atgtcaccat tgaagagttc aacgctgttg cgcatgaact
0771 ggacccgatc gtggcaaaac gcgtgcgtca tatactgact gaaaacgccc gcaccgttga agctgccagc
0841 gcgctggagc aaggcgacct gaaacgtatg ggcgagttga tggcggagtc tcatgcctct atgcgcgatg
0911 atttcgaaat caccgtgccg caaattgaca ctctggtaga aatcgtcaaa gctgtgattg gcgacaaagg
0981 tggcgtacgc atgaccggcg gcggatttgg cggctgtatc gtcgcgctga tcccggaaga gctggtgcct
1051 gccgtacagc aagctgtcgc tgaacaatat gaagcaaaaa caggtattaa agagactttt tacgtttgta
1121 aaccatcaca aggagcagga cagtgctgaa cgaaactccc gcactggcac ccgatggtca gccgtaccga
1191 ctgttaactt tgcgtaacaa cgcagggatg gtagtcacgc tgatggactg gggtgcgac
|
