|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Acta Microbiol Pol. 1997;46(1):7-17.
Escherichia coli defects caused by null mutations in dnaK and dnaJ genes.
Paciorek J, Kardys K, Lobacz B, Wolska KI.
(
9271843
)
|
|
|
Deletion
|
OCR27
(D)
,
OCR27-6
(D)
,
OCR27-7
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
dnaK
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
dnaK
Search strains by all related name:
dnaK b0014 ECK0014 gro groP groPAB groPC groPF grpC grpF JW0013 seg
|
General information (Go to Linear View: )
|
|
Gene Name
|
dnaK
|
|
Alternative name
|
b0014,ECK0014,gro,groP,groPAB,groPC,groPF,grpC,grpF,JW0013,seg
|
|
Location, Length
|
12,163 - 14,079
(
+
)
;
0.26
min
;
1917
(bp) ,
638
(aa)
|
|
Product
|
chaperone Hsp70, co-chaperone with DnaJ
|
|
Operon Name
|
dnaK-tpke11-dnaJ
|
|
Note
|
chaperone Hsp70; DNA biosynthesis; autoregulated heat shock proteins; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006457 - protein folding; GO_process: GO:0006970 - response to osmotic stress
|
|
Function
|
factor; Chaperones,2.3.4 information transfer; protein related; chaperoning, folding,5.5.1 cell processes; adaptation to stress; osmotic pressure,7.1 location of gene products; cytoplasm
|
|
Gene Ontology
|
GO:0000166
;
nucleotide binding ( dnaK )
GO:0005515
;
protein binding ( dnaK )
GO:0005524
;
ATP binding ( dnaK )
GO:0005737
;
cytoplasm ( dnaK )
GO:0005886
;
plasma membrane ( dnaK )
GO:0006260
;
DNA replication ( dnaK )
GO:0006457
;
protein folding ( dnaK )
GO:0006950
;
response to stress ( dnaK )
GO:0016020
;
membrane ( dnaK )
GO:0051082
;
unfolded protein binding ( dnaK )
|
|
PID
|
1786196
|
EC number
(KEGG Pathway)
|
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
dnaK
(
EG10241
)
|
|
Number of removed
|
1 aa cleaved
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
DNAB_ECOLI
(
P03005
)
|
|
- Protein name
|
Replicative DNA helicase
|
|
- Synonyms
|
EC 3.6.1.-
|
|
- Gene name
|
OrderedLocusNames=b4052, SF4154, S3577;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
dnaK
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Crystal Structure Of Bovine Hsc70
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Chaperone protein dnaK (Heat shock protein 70) (Heat shock 70 kDa protein) (HSP70)
|
|
PSI-BLAST
|
Chaperone protein dnaK2
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
molecular chaperone DnaK [Escherichia coli O157:H7 EDL933]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
AMIDATION
HSP70_1
HSP70_2
HSP70_3
|
|
MMBR References
|
Microbiology 1996;142:2595-2602
Defect in export and synthesis of the periplasmic galactose receptor MglB in dnaK mutants of Escherichia coli, and decreased stability of the mglB mRNA.
el Yaagoubi, A., M. Kohiyama, and G. Richarme.
|
Mol Gen Genet. 1990;223(3):361-8.
Possible involvement of the ugpA gene product in the stable maintenance of mini-F plasmid in Escherichia coli.
Ezaki B, Mori H, Ogura T, Hiraga S.
(
2270076
)
|
Mol Gen Genet. 1989;218(2):183-9.
Involvement of DnaK protein in mini-F plasmid replication: temperature-sensitive seg mutations are located in the dnaK gene.
Ezaki B, Ogura T, Mori H, Niki H, Hiraga S.
(
2674651
)
|
J Biol Chem. 1990;265:19244-19248
Aggregated dnaA protein is dissociated and activated for DNA replication by phospholipase or dnaK protein.
Hwang, D. S., E. Crooke, and A. Kornberg.
|
J Bacteriol. 1994;176:5648-5653
Induction of heat shock proteins by abnormal proteins results from stabilization and not increased synthesis of sigma32 in Escherichia coli.
Kanemori, M., H. Mori, and T. Yura.
|
Proc Natl Acad Sci U S A. 1984;81:848-852
Major heat shock gene of Drosophila and the Escherichia coli heat-inducible dnaK gene are homologous.
Bardwell, J. C., and E. A. Craig.
|
J Bacteriol. 1994;176:764-780
DnaK mutants defective in ATPase activity are defective in negative regulation of the heat shock response: expression of mutant DnaK proteins results in filamentation.
McCarty, J. S., and G. C. Walker.
|
J Bacteriol. 1997;179:445-452
Heat shock regulation of sigma S turnover: a role for DnaK and relationship between stress responses mediated by sigma S and sigma 32 in Escherichia coli.
Muffler, A., M. Barth, C. Marschall, and R. Hengge-Aronis.
|
Eur J Biochem. 1996;240:78-82
Nucleotide binding to the heat-shock protein DnaK as studied by ESR spectroscopy.
Neuhofen, S., H. Theyssen, J. Reinstein, W. E. Trommer, and P. D. Vogel.
|
Biochim Biophys Acta. 1989;1009(1):94-8.
A complete deletion mutant of the Escherichia coli dnaKdnaJ operon.
Ohki R, Morita R, Kawamata T, Uchida H, Ohki M.
(
2675977
)
|
J Bacteriol. 1987;169(1):283-90.
Escherichia coli dnaK null mutants are inviable at high temperature.
Paek KH, Walker GC.
(
3025174
)
|
J Bacteriol. 1998;180:846-854
Roles of DnaK and RpoS in starvation-induced thermotolerance of Escherichia coli.
Rockabrand, D., K. Livers, T. Austin, R. Kaiser, D. Jensen, R. Burgess, and P. Blum.
|
J Bacteriol. 1988;170(2):972-9.
The dnaK gene of Escherichia coli functions in initiation of chromosome replication.
Sakakibara Y.
(
2962992
)
|
Genes Dev. 1990;4:2202-2209
DnaK, DnaJ, and GrpE heat shock proteins negatively regulate heat shock gene expression by controlling the synthesis and stability of sigma 32.
Straus, D., W. Walter, and C. A. Gross.
|
Proc Natl Acad Sci U S A. 1996;93:10632-10637
Mutations in the C-terminal fragment of DnaK affecting peptide binding.
Burkholder, W. F., X. Zhao, X. Zhu, W. A. Hendrickson, A. Gragerov, and M. E. Gottesman.
|
| Sequences |
Amino acid
FASTA format
|
0001 MGKIIGIDLG TTNSCVAIMD GTTPRVLENA EGDRTTPSII AYTQDGETLV GQPAKRQAVT NPQNTLFAIK
0071 RLIGRRFQDE EVQRDVSIMP FKIIAADNGD AWVEVKGQKM APPQISAEVL KKMKKTAEDY LGEPVTEAVI
0141 TVPAYFNDAQ RQATKDAGRI AGLEVKRIIN EPTAAALAYG LDKGTGNRTI AVYDLGGGTF DISIIEIDEV
0211 DGEKTFEVLA TNGDTHLGGE DFDSRLINYL VEEFKKDQGI DLRNDPLAMQ RLKEAAEKAK IELSSAQQTD
0281 VNLPYITADA TGPKHMNIKV TRAKLESLVE DLVNRSIEPL KVALQDAGLS VSDIDDVILV GGQTRMPMVQ
0351 KKVAEFFGKE PRKDVNPDEA VAIGAAVQGG VLTGDVKDVL LLDVTPLSLG IETMGGVMTT LIAKNTTIPT
0421 KHSQVFSTAE DNQSAVTIHV LQGERKRAAD NKSLGQFNLD GINPAPRGMP QIEVTFDIDA DGILHVSAKD
0491 KNSGKEQKIT IKASSGLNED EIQKMVRDAE ANAEADRKFE ELVQTRNQGD HLLHSTRKQV EEAGDKLPAD
0561 DKTAIESALT ALETALKGED KAAIEAKMQE LAQVSQKLME IAQQQHAQQQ TAGADASANN AKDDDVVDAE
0631 FEEVKDKK
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 GTCTGCAAAA AAATGAAATT GGGCAGTTGA
-070 AACCAGACGT TTCGCCCCTA TTACAGACTC ACAACCACAT GATGACCGAA TATATAGTGG AGACGTTTAG
0001 atgggtaaaa taattggtat cgacctgggt actaccaact cttgtgtagc gattatggat ggcaccactc
0071 ctcgcgtgct ggagaacgcc gaaggcgatc gcaccacgcc ttctatcatt gcctataccc aggatggtga
0141 aactctagtt ggtcagccgg ctaaacgtca ggcagtgacg aacccgcaaa acactctgtt tgcgattaaa
0211 cgcctgattg gtcgccgctt ccaggacgaa gaagtacagc gtgatgtttc catcatgccg ttcaaaatta
0281 ttgctgctga taacggcgac gcatgggtcg aagttaaagg ccagaaaatg gcaccgccgc agatttctgc
0351 tgaagtgctg aaaaaaatga agaaaaccgc tgaagattac ctgggtgaac cggtaactga agctgttatc
0421 accgtaccgg catactttaa cgatgctcag cgtcaggcaa ccaaagacgc aggccgtatc gctggtctgg
0491 aagtaaaacg tatcatcaac gaaccgaccg cagctgcgct ggcttacggt ctggacaaag gcactggcaa
0561 ccgtactatc gcggtttatg acctgggtgg tggtactttc gatatttcta ttatcgaaat cgacgaagtt
0631 gacggcgaaa aaaccttcga agttctggca accaacggtg atacccacct ggggggtgaa gacttcgaca
0701 gccgtctgat caactatctg gttgaagaat tcaagaaaga tcagggcatt gacctgcgca acgatccgct
0771 ggcaatgcag cgcctgaaag aagcggcaga aaaagcgaaa atcgaactgt cttccgctca gcagaccgac
0841 gttaacctgc catacatcac tgcagacgcg accggtccga aacacatgaa catcaaagtg actcgtgcga
0911 aactggaaag cctggttgaa gatctggtaa accgttccat tgagccgctg aaagttgcac tgcaggacgc
0981 tggcctgtcc gtatctgata tcgacgacgt tatcctcgtt ggtggtcaga ctcgtatgcc aatggttcag
1051 aagaaagttg ctgagttctt tggtaaagag ccgcgtaaag acgttaaccc ggacgaagct gtagcaatcg
1121 gtgctgctgt tcagggtggt gttctgactg gtgacgtaaa agacgtactg ctgctggacg ttaccccgct
1191 gtctctgggt atcgaaacca tgggcggtgt gatgacgacg ctgatcgcga aaaacaccac tatcccgacc
1261 aagcacagcc aggtgttctc taccgctgaa gacaaccagt ctgcggtaac catccatgtg ctgcagggtg
1331 aacgtaaacg tgcggctgat aacaaatctc tgggtcagtt caacctagat ggtatcaacc cggcaccgcg
1401 cggcatgccg cagatcgaag ttaccttcga tatcgatgct gacggtatcc tgcacgtttc cgcgaaagat
1471 aaaaacagcg gtaaagagca gaagatcacc atcaaggctt cttctggtct gaacgaagat gaaatccaga
1541 aaatggtacg cgacgcagaa gctaacgccg aagctgaccg taagtttgaa gagctggtac agactcgcaa
1611 ccagggcgac catctgctgc acagcacccg taagcaggtt gaagaagcag gcgacaaact gccggctgac
1681 gacaaaactg ctatcgagtc tgcgctgact gcactggaaa ctgctctgaa aggtgaagac aaagccgcta
1751 tcgaagcgaa aatgcaggaa ctggcacagg tttcccagaa actgatggaa atcgcccagc agcaacatgc
1821 ccagcagcag actgccggtg ctgatgcttc tgcaaacaac gcgaaagatg acgatgttgt cgacgctgaa
1891 tttgaagaag tcaaagacaa aaaataaTCG CCCTATAAAC GGGTAATTAT ACTGACACGG GCGAAGGGGA
1961 ATTTCCTCTC CGCCCGTGCA TTCATCTAGG GGCAATTTAA AAAAGATGGC TAAGCAA
|
