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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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J Bacteriol. 1978;135(2):429-35.
Regulation of the synthesis of ribonucleoside diphosphate reductase in Escherichia coli: specific activity of the enzyme in relationship to perturbations of DNA replication.
Filpula D, Fuchs JA.
(
355225
)
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Deletion
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OCR33,35-4 (AK4-2 / BK5-2)
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
dnaE
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Related strains
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Strains Search
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Search strains by gene name:
dnaE
Search strains by all related name:
dnaE b0184 ECK0183 JW0179 polC sdgC
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General information (Go to Linear View: )
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Gene Name
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dnaE
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Alternative name
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b0184,ECK0183,JW0179,polC,sdgC
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Location, Length
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205,126 - 208,608
(
+
)
;
4.42
min
;
3483
(bp) ,
1160
(aa)
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Product
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DNA polymerase III alpha subunit
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Operon Name
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bamA-hlpA-lpxD-fabZ-lpxAB-rnhB-dnaE
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Note
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DNA polymerase III, alpha subunit; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006261 - DNA-dependent DNA replication
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Function
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enzyme; DNA - replication, repair,restriction/modification
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Gene Ontology
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GO:0003676
;
nucleic acid binding ( dnaE )
GO:0003677
;
DNA binding ( dnaE )
GO:0003824
;
catalytic activity ( dnaE )
GO:0003887
;
DNA-directed DNA polymerase activity ( dnaE )
GO:0003889
;
alpha DNA polymerase activity ( dnaE )
GO:0005515
;
protein binding ( dnaE )
GO:0005737
;
cytoplasm ( dnaE )
GO:0006260
;
DNA replication ( dnaE )
GO:0008408
;
3'-5' exonuclease activity ( dnaE )
GO:0016740
;
transferase activity ( dnaE )
GO:0016779
;
nucleotidyltransferase activity ( dnaE )
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PID
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1786381
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EC number
(KEGG Pathway)
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2.7.7.7
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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DP3A_ECOLI
(
P10443
)
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- Protein name
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DNA polymerase III alpha subunit
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- Synonyms
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EC 2.7.7.7
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- Gene name
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Name=dnaE; Synonyms=polC; OrderedLocusNames=b0184;
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MMBR References
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J Bacteriol. 1997;179:7435-7445
Genetic requirements and mutational specificity of the Escherichia coli SOS mutator activity.
Fijalkowska, I. J., R. L. Dunn, and R. M. Schaaper.
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J Bacteriol. 1995;177:5979-5986
Effects of Escherichia coli dnaE antimutator alleles in a proofreading-deficient mutD5 strain.
Fijalkowska, I. J., and R. M. Schaaper.
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Annu Rev Biochem. 1995;64:171-200.
DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine.
Kelman Z, O'Donnell M.
(
7574479
)
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Mol Gen Genet. 1981;181(3):356-62.
Organization of genes in the four minute region of the Escherichia coli chromosome: evidence that rpsB and tsf are co-transcribed.
Bendiak DS, Friesen JD.
(
7017348
)
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Mutat Res. 1996;350(1):17-23.
Suppressors of Escherichia coli mutT: antimutators for DNA replication errors.
Schaaper RM.
(
8657178
)
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J Bacteriol. 1984;158(2):455-9.
Determination of the precise location and orientation of the Escherichia coli dnaE gene.
Shepard D, Oberfelder RW, Welch MM, McHenry CS.
(
6327605
)
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J Bacteriol. 1987;169(12):5735-44.
Sequence analysis of the Escherichia coli dnaE gene.
Tomasiewicz HG, McHenry CS.
(
3316192
)
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J Bacteriol. 1982;152(1):351-6.
Cloning and identification of the product of the dnaE gene of Escherichia coli.
Welch MM, McHenry CS.
(
6288664
)
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J Bacteriol. 1992;174:4166-4168
Overproduction of DnaE protein ( subunit of DNA polymerase III) restores viability in a conditionally inviable Escherichia coli strain deficient in DNA polymerase I.
Witkin, E. M., and V. Roegner-Maniscalco.
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| Sequences |
Amino acid
FASTA format
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0001 MSEPRFVHLR VHSDYSMIDG LAKTAPLVKK AAALGMPALA ITDFTNLCGL VKFYGAGHGA GIKPIVGADF
0071 NVQCDLLGDE LTHLTVLAAN NTGYQNLTLL ISKAYQRGYG AAGPIIDRDW LIELNEGLIL LSGGRMGDVG
0141 RSLLRGNSAL VDECVAFYEE HFPDRYFLEL IRTGRPDEES YLHAAVELAE ARGLPVVATN DVRFIDSSDF
0211 DAHEIRVAIH DGFTLDDPKR PRNYSPQQYM RSEEEMCELF ADIPEALANT VEIAKRCNVT VRLGEYFLPQ
0281 FPTGDMSTED YLVKRAKEGL EERLAFLFPD EEERLKRRPE YDERLETELQ VINQMGFPGY FLIVMEFIQW
0351 SKDNGVPVGP GRGSGAGSLV AYALKITDLD PLEFDLLFER FLNPERVSMP DFDVDFCMEK RDQVIEHVAD
0421 MYGRDAVSQI ITFGTMAAKA VIRDVGRVLG HPYGFVDRIS KLIPPDPGMT LAKAFEAEPQ LPEIYEADEE
0491 VKALIDMARK LEGVTRNAGK HAGGVVIAPT KITDFAPLYC DEEGKHPVTQ FDKSDVEYAG LVKFDFLGLR
0561 TLTIINWALE MINKRRAKNG EPPLDIAAIP LDDKKSFDML QRSETTAVFQ LESRGMKDLI KRLQPDCFED
0631 MIALVALFRP GPLQSGMVDN FIDRKHGREE ISYPDVQWQH ESLKPVLEPT YGIILYQEQV MQIAQVLSGY
0701 TLGGADMLRR AMGKKKPEEM AKQRSVFAEG AEKNGINAEL AMKIFDLVEK FAGYGFNKSH SAAYALVSYQ
0771 TLWLKAHYPA EFMAAVMTAD MDNTEKVVGL VDECWRMGLK ILPPDINSGL YHFHVNDDGE IVYGIGAIKG
0841 VGEGPIEAII EARNKGGYFR ELFDLCARTD TKKLNRRVLE KLIMSGAFDR LGPHRAALMN SLGDALKAAD
0911 QHAKAEAIGQ ADMFGVLAEE PEQIEQSYAS CQPWPEQVVL DGERETLGLY LTGHPINQYL KEIERYVGGV
0981 RLKDMHPTER GKVITAAGLV VAARVMVTKR GNRIGICTLD DRSGRLEVML FTDALDKYQQ LLEKDRILIV
1051 SGQVSFDDFS GGLKMTAREV MDIDEAREKY ARGLAISLTD RQIDDQLLNR LRQSLEPHRS GTIPVHLYYQ
1121 RADARARLRF GATWRVSPSD RLLNDLRGLI GSEQVELEFD
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 CGCGACCGAA CACCATCGGC GCAGCTTTGG
-070 GCCTGTCAAA CGCGCACTGG GACTTGCGTC CTGATTCTTG TGTCGAGATT AAGTAAACCG GAATCTGAAG
0001 atgtctgaac cacgtttcgt acacctgcgg gtgcacagcg actactcgat gatcgatggc ctggccaaaa
0071 ccgcaccgtt ggtaaaaaag gcggcggcgt tgggtatgcc agcactggcg atcaccgatt tcaccaacct
0141 ttgtggtctg gtgaagttct acggagcggg acatggcgca gggattaagc ctatcgtcgg ggcagatttt
0211 aacgtccagt gcgacctgct gggtgatgag ttaacccacc tgacggtact ggcggcgaac aataccggct
0281 atcagaatct gacgttgctg atctcaaaag cgtatcagcg cgggtacggt gccgccgggc cgatcatcga
0351 tcgcgactgg cttatcgaat taaacgaagg gttgatcctt ctttccggcg gacgcatggg cgacgtcgga
0421 cgcagtcttt tgcgtggtaa cagcgcgctg gtagatgagt gtgtcgcgtt ttatgaagaa cacttcccgg
0491 atcgctattt tctcgagctg atccgcaccg gcaggccgga tgaagaaagc tatctgcacg cggcggtgga
0561 actggcggaa gcgcgcggtt tgcccgtcgt ggcgaccaac gacgtgcgct ttatcgacag cagcgacttt
0631 gacgcacacg aaatccgcgt cgcgatccac gacggcttta ccctcgacga tcctaaacgc ccgcgtaact
0701 attcgccgca gcaatatatg cgtagcgaag aggagatgtg tgagctgttt gccgacatcc ccgaagccct
0771 tgccaacacc gttgagatcg ccaaacgctg taacgtaacc gtgcgtcttg gtgaatactt cctgccgcag
0841 ttcccgaccg gggacatgag caccgaagat tatctggtca agcgtgcaaa agagggcctg gaagagcgtc
0911 tggccttttt attccctgat gaggaagaac gtcttaagcg ccgcccggaa tatgacgaac gtctggagac
0981 tgaacttcag gttatcaacc agatgggctt cccgggctac ttcctcatcg ttatggaatt tatccagtgg
1051 tcgaaagata acggcgtacc ggtagggcca ggccgtggct ccggtgcggg ttcactggtg gcctacgcgc
1121 tgaaaatcac cgacctcgat ccgctggaat ttgacctgct gttcgaacgt ttccttaacc cggaacgtgt
1191 ctccatgcct gacttcgacg ttgacttctg tatggagaaa cgcgatcagg ttatcgagca cgtagcggac
1261 atgtacggtc gtgatgcggt atcgcagatc atcaccttcg gtacaatggc ggcgaaagcg gtgatccgcg
1331 acgtaggccg cgtgctgggg catccgtacg gctttgtcga tcgtatctcg aaactgatcc cgcccgatcc
1401 ggggatgacg ctggcgaaag cgtttgaagc cgagccgcag ctgccggaaa tctacgaagc ggatgaagaa
1471 gttaaggcgc tgatcgacat ggcgcgcaaa ctggaagggg tcacccgtaa cgccggtaag cacgccggtg
1541 gggtggttat cgcgccgacc aaaattaccg attttgcgcc gctttactgc gatgaagagg gcaaacatcc
1611 ggtcacccag tttgataaaa gcgacgttga atacgccgga ctggtgaagt tcgacttcct tggtttgcgt
1681 acgctcacca tcatcaactg ggcgctggag atgatcaaca agcggcgggc gaagaatggc gagccgccgc
1751 tggatatcgc tgcgatcccg ctggatgata agaaaagctt cgacatgctg caacgctcgg aaaccacggc
1821 ggtattccag cttgaatcgc gcggcatgaa ggacctgatc aagcgtctac aacctgactg cttcgaagat
1891 atgatcgccc tagtggcact gttccgcccc ggtccgttgc aatcagggat ggtggataac tttatcgacc
1961 gtaaacatgg tcgtgaagag atctcctatc cggacgtaca gtggcagcat gaaagcctga aaccggtact
2031 ggagccaacc tacggcatta tcctgtatca ggaacaggtc atgcagattg cgcaggtgct ttctggttat
2101 accctcggtg gcgcggatat gctgcgtcgt gcgatgggta agaaaaagcc ggaagagatg gctaagcaac
2171 gttctgtatt tgctgaaggt gcagaaaaga acggaatcaa cgctgaactg gcgatgaaaa tcttcgacct
2241 ggtggagaaa ttcgctggtt acggatttaa caaatcgcac tctgcggcct atgctttggt gtcatatcaa
2311 acgttatggc tgaaagcgca ctatcctgcg gagtttatgg cggcggtaat gaccgccgat atggacaaca
2381 ccgagaaggt ggtgggtctg gtggatgagt gctggcggat ggggctgaaa atcctgccac cagatataaa
2451 ctccggtctt taccatttcc acgtcaacga cgacggcgaa atcgtgtatg gtattggcgc gatcaaaggg
2521 gtcggtgaag gtccgattga ggccatcatc gaagcccgta ataaaggcgg ctacttccgc gaactgtttg
2591 atctctgcgc ccgtaccgac accaaaaagt tgaaccgtcg cgtgctggaa aaactgatca tgtccggggc
2661 gtttgaccgt cttgggccac atcgcgcagc gctgatgaac tcgctgggcg atgcgttaaa agcggcagat
2731 caacacgcga aagcggaagc tatcggtcag gccgatatgt tcggcgtgct ggccgaagag ccggaacaaa
2801 ttgaacaatc ctacgccagc tgccaaccgt ggccggagca ggtggtatta gatggggaac gtgaaacgtt
2871 aggcctgtac ctgaccggac accctatcaa ccagtattta aaagagattg agcgttatgt cggaggcgta
2941 aggctgaaag acatgcaccc gacagaacgt ggtaaagtca tcacggctgc ggggctcgtt gttgccgcgc
3011 gggttatggt caccaagcgc ggcaatcgta tcggtatctg cacgctggat gaccgttccg ggcggctgga
3081 agtgatgttg tttactgacg ccctggataa ataccagcaa ttgctggaaa aagaccgcat acttatcgtc
3151 agcggacagg tcagctttga tgacttcagc ggtgggctta aaatgaccgc tcgcgaagtg atggatattg
3221 acgaagcccg ggaaaaatat gctcgcgggc ttgctatctc gctgacggac aggcaaattg atgaccagct
3291 tttaaaccga ctccgtcagt ctctggaacc ccaccgctct gggacaattc cagtacatct ctactatcag
3361 agggcggatg cacgcgcgcg gttgcgtttt ggcgcgacgt ggcgtgtctc tccgagcgat cgtttattaa
3431 acgatctccg tggcctcatt ggttcggagc aggtggaact ggagtttgac taaTACAGGA ATACTATGAG
3501 TCTGAATTTC CTTGATTTTG AACAGCCGAT TGCAGAGCTG GAAGCGAAAA TCGATTCTCT GACTGCGGTT
3571 AGCCGTCAGG ATG
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