PEC Original Annotations
|
Essentiality
|
Class
|
non-essential
|
References (PMID)
|
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
|
|
Deletion
|
OCR30,31-3 (BK2-1 / AK1-1)
(D)
|
Related gene (W3110 PEC)
|
Gene Search
|
Search MG1655 PEC by gene name:
ddlB
|
Related strains
|
Strains Search
|
Search strains by gene name:
ddlB
Search strains by all related name:
ddlB b0092 ddl ECK0093 JW0090
|
General information (Go to Linear View:)
|
Gene Name
|
ddlB
|
Alternative name
|
b0092,ddl,ECK0093,JW0090
|
Location, Length
|
102,233 - 103,153
(
+
)
;
2.20
min
;
921
(bp) ,
306
(aa)
|
Product
|
D-alanine:D-alanine ligase
|
Operon Name
|
mraZ-rsmH-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ-lpxC
|
Note
|
D-alanine-D-alanine ligase B, affects cell division; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009252 - peptidoglycan biosynthetic process
|
Function
|
enzyme; Murein sacculus, peptidoglycan
|
Gene Ontology
|
GO:0000166
;
nucleotide binding ( ddlB )
GO:0003824
;
catalytic activity ( ddlB )
GO:0005524
;
ATP binding ( ddlB )
GO:0005618
;
cell wall ( ddlB )
GO:0005737
;
cytoplasm ( ddlB )
GO:0007047
;
cell wall organization and biogenesis ( ddlB )
GO:0008360
;
regulation of cell shape ( ddlB )
GO:0008716
;
D-alanine-D-alanine ligase activity ( ddlB )
GO:0009252
;
peptidoglycan biosynthetic process ( ddlB )
GO:0016874
;
ligase activity ( ddlB )
GO:0030145
;
manganese ion binding ( ddlB )
GO:0046872
;
metal ion binding ( ddlB )
|
PID
|
1786280
|
EC number
(KEGG Pathway)
|
6.3.2.4
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
EcoGene
|
ddlB
(
EG10214
)
|
Number of removed
|
1 aa cleaved
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
DDLB_ECOLI
(
P07862
)
|
- Protein name
|
D-alanine--D-alanine ligase B
|
- Synonyms
|
EC 6.3.2.4, D-alanylalanine synthetase B, D-Ala-D-Ala ligase B
|
- Gene name
|
Name=ddlB; Synonyms=ddl; OrderedLocusNames=b0092;
|
Homology Analysis
|
BLAST
|
Bacteria
|
GTOP
|
ddlB
(
homologous genes of other bacterias
)
|
PDB
(database updated :
2007.02.20
)
|
PSI-BLAST
|
Chain , Mol_id: 1; Molecule: D-Alanine--D-Alanine Ligase; Chain: Null; Ec: 6.3.2.4
|
SWISS-PROT
(database updated :
2007.02.20
)
|
BLAST
|
D-alanine--D-alanine ligase B
|
PSI-BLAST
|
D-alanine--D-alanine ligase
|
nr
(database updated :
2007.02.20
)
|
BLAST
|
D-alanylalanine synthetase [Escherichia coli K12]
|
Pfam 28.0
(database updated :
2015-05
)
|
Pfam
|
|
PROSITE
|
PROSITE
|
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
GRAM_POS_ANCHORING
DALA_DALA_LIGASE_1
DALA_DALA_LIGASE_2
|
MMBR References
|
Nucleic Acids Res. 1990;18(13):4014
Nucleotide sequence involving murG and murC in the mra gene cluster region of Escherichia coli.
Ikeda M, Wachi M, Jung HK, Ishino F, Matsuhashi M.
(
2197603
)
|
J Bacteriol. 1989;171(11):6379-82.
Inhibition of growth of ftsQ, ftsA, and ftsZ mutant cells of Escherichia coli by amplification of a chromosomal region encompassing closely aligned cell division and cell growth genes.
Jung HK, Ishino F, Matsuhashi M.
(
2553679
)
|
J Bacteriol. 1980;142(2):615-20.
Organization of genes in the ftsA-envA region of the Escherichia coli genetic map and identification of a new fts locus (ftsZ).
Lutkenhaus JF, Wolf-Watz H, Donachie WD.
(
6991482
)
|
J Bacteriol. 1980;143(3):1281-8.
Determination of transcriptional units and gene products from the ftsA region of Escherichia coli.
Lutkenhaus JF, Wu HC.
(
6447690
)
|
Biochemistry. 1996;35(32):10464-71.
Gain of D-alanyl-D-lactate or D-lactyl-D-alanine synthetase activities in three active-site mutants of the Escherichia coli D-alanyl-D-alanine ligase B.
Park IS, Lin CH, Walsh CT.
(
8756703
)
|
J Bacteriol. 1986;167(3):809-17.
Further evidence for overlapping transcriptional units in an Escherichia coli cell envelope-cell division gene cluster: DNA sequence and transcriptional organization of the ddl ftsQ region.
Robinson AC, Kenan DJ, Sweeney J, Donachie WD.
(
3528126
)
|
Sequences |
Amino acid
FASTA format
|
0001 MTDKIAVLLG GTSAEREVSL NSGAAVLAGL REGGIDAYPV DPKEVDVTQL KSMGFQKVFI ALHGRGGEDG
0071 TLQGMLELMG LPYTGSGVMA SALSMDKLRS KLLWQGAGLP VAPWVALTRA EFEKGLSDKQ LAEISALGLP
0141 VIVKPSREGS SVGMSKVVAE NALQDALRLA FQHDEEVLIE KWLSGPEFTV AILGEEILPS IRIQPSGTFY
0211 DYEAKYLSDE TQYFCPAGLE ASQEANLQAL VLKAWTTLGC KGWGRIDVML DSDGQFYLLE ANTSPGMTSH
0281 SLVPMAARQA GMSFSQLVVR ILELAD
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 AACGACCTGA TTCTCGTTCA GGGGGCTGGT
-070 AATATTGGAA AAATTGCCCG TTCTTTAGCT GAAATCAAAC TGAAGCCGCA AACTCCGGAG GAAGAACAAC
0001 atgactgata aaatcgcggt cctgttgggt gggacctccg ctgagcggga agtttctctg aattctggcg
0071 cagcggtgtt agccggactg cgtgaaggcg gtattgacgc gtatcctgtc gacccgaaag aagtcgacgt
0141 gacgcaactg aagtcgatgg gctttcagaa agtgtttatc gcgctacacg gtcgcggcgg tgaagatggt
0211 acgctgcagg ggatgctcga gctgatgggc ttgccttata ccggaagcgg agtgatggca tctgcgcttt
0281 caatggataa actacgcagc aaacttctat ggcaaggtgc cggtttaccg gtcgcgccgt gggtagcgtt
0351 aacccgcgca gagtttgaaa aaggcctgag cgataagcag ttagcagaaa tttctgctct gggtttgccg
0421 gttatcgtta agccgagccg cgaaggttcc agtgtgggaa tgtcaaaagt agtagcagaa aatgctctac
0491 aagatgcatt aagattggca tttcagcacg atgaagaagt attgattgaa aaatggctaa gtgggccgga
0561 gttcacggtt gcgatactcg gtgaagaaat tttaccgtca atacgtattc aaccgtccgg aaccttctat
0631 gattatgagg cgaagtatct ctctgatgag acacagtatt tctgccccgc aggtctggaa gcgtcacaag
0701 aggccaattt gcaggcatta gtgctgaaag catggacgac gttaggttgc aaaggatggg gacgtattga
0771 cgttatgctg gacagcgatg gacagtttta tctgctggaa gccaatacct caccgggtat gaccagccac
0841 agcctggtgc cgatggcggc acgtcaggca ggtatgagct tctcgcagtt ggtagtacga attctggaac
0911 tggcggacta aTATGTCGCA GGCTGCTCTG AACACGCGAA ACAGCGAAGA AGAGGTTTCT TCTCGCCGCA
0981 ATAATGGAAC GCGTCTGGCG GGGATCCTTT TCCTGCTGAC C
|