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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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J Bacteriol. 1980;144(3):1190-2.
Deletion of the penicillin-binding protein 5 gene of Escherichia coli.
Spratt BG.
(
7002911
)
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Deletion
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OCR46
(D)
,
OCR46-10
(D)
,
OCR46-11
(D)
,
OCR46-8
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
dacA
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Related strains
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Strains Search
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Search strains by gene name:
dacA
Search strains by all related name:
dacA b0632 ECK0625 JW0627 pfv
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General information (Go to Linear View: )
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Gene Name
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dacA
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Alternative name
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b0632,ECK0625,JW0627,pfv
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Location, Length
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661,975 - 663,186
(
-
)
;
14.27
min
;
1212
(bp) ,
403
(aa)
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Product
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D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5)
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Operon Name
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dacA
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Note
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D-alanyl-D-alanine carboxypeptidase, fraction A; penicillin-binding protein 5; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0042493 - response to drug
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Function
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enzyme; Murein sacculus, peptidoglycan
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Gene Ontology
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GO:0004180
;
carboxypeptidase activity ( dacA )
GO:0005515
;
protein binding ( dacA )
GO:0005886
;
plasma membrane ( dacA )
GO:0006508
;
proteolysis ( dacA )
GO:0007047
;
cell wall organization and biogenesis ( dacA )
GO:0008233
;
peptidase activity ( dacA )
GO:0008360
;
regulation of cell shape ( dacA )
GO:0008800
;
beta-lactamase activity ( dacA )
GO:0009002
;
serine-type D-Ala-D-Ala carboxypeptidase activity ( dacA )
GO:0009252
;
peptidoglycan biosynthetic process ( dacA )
GO:0016020
;
membrane ( dacA )
GO:0016787
;
hydrolase activity ( dacA )
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PID
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1786851
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EC number
(KEGG Pathway)
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3.4.16.4
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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dacA
(
EG10201
)
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Number of removed
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29 aa cleaved
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SWISS-PROT
(
Show details
)
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Entry name(Acc.no)
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DACA_ECOLI
(
P04287
;
P77106
)
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- Protein name
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Penicillin-binding protein 5 precursor
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- Synonyms
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D-alanyl-D-alanine carboxypeptidase fraction A, EC 3.4.16.4, DD-peptidase, DD- carboxypeptidase, PBP-5
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- Gene name
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OrderedLocusNames=b0632, c0722, z0777, ECs0670;
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MMBR References
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Proc Natl Acad Sci U S A. 1978;75:2631-2635
Isolation of a mutant of Escherichia coli lacking penicillin-sensitive D-alanine carboxypeptidase IA.
Matsuhashi, M., I. N. Maruyama, Y. Takagaki, S. Tamaki, Y. Nishimura, and Y. Hirota.
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J Bacteriol. 1979;137(1):644-7.
Mutational evidence for identity of penicillin-binding protein 5 in Escherichia coli with the major D-alanine carboxypeptidase IA activity.
Matsuhashi M, Tamaki S, Curtis SJ, Strominger JL.
(
368033
)
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J Bacteriol. 1980;143(1):531-4.
A mutant of Escherichia coli defective in penicillin-binding protein 5 and lacking D-alanine carboxypeptidase IA.
Nishimura Y, Suzuki H, Hirota Y, Park JT.
(
6995448
)
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Agric. Biol. Chem. 1985;49:881-883.
Cloning of the gene of Escherichia coli mutant penicillin-binding protein 5 that has no penicillin-releasing activity.
Park, W., I. Takase, S. Tamaki, I.-J. Jin, F. Ishino, and M. Matsuhashi.
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J Bacteriol. 1980;143(2):569-81.
Defective and plaque-forming lambda transducing bacteriophage carrying penicillin-binding protein-cell shape genes: genetic and physical mapping and identification of gene products from the lip-dacA-rodA-pbpA-leuS region of the Escherichia coli chromosome.
Spratt BG, Boyd A, Stoker N.
(
6451612
)
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J Bacteriol. 1983;155(2):847-53.
Organization and subcloning of the dacA-rodA-pbpA cluster of cell shape genes in Escherichia coli.
Stoker NG, Broome-Smith JK, Edelman A, Spratt BG.
(
6348028
)
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Nucleic Acids Res. 1988;16(4):1617
Nucleotide sequences of the penicillin-binding protein 5 and 6 genes of Escherichia coli.
Broome-Smith JK, Ioannidis I, Edelman A, Spratt BG.
(
3279397
)
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| Sequences |
Amino acid
FASTA format
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0001 MNTIFSARIM KRLALTTALC TAFISAAHAD DLNIKTMIPG VPQIDAESYI LIDYNSGKVL AEQNADVRRD
0071 PASLTKMMTS YVIGQAMKAG KFKETDLVTI GNDAWATGNP VFKGSSLMFL KPGMQVPVSQ LIRGINLQSG
0141 NDACVAMADF AAGSQDAFVG LMNSYVNALG LKNTHFQTVH GLDADGQYSS ARDMALIGQA LIRDVPNEYS
0211 IYKEKEFTFN GIRQLNRNGL LWDNSLNVDG IKTGHTDKAG YNLVASATEG QMRLISAVMG GRTFKGREAE
0281 SKKLLTWGFR FFETVNPLKV GKEFASEPVW FGDSDRASLG VDKDVYLTIP RGRMKDLKAS YVLNSSELHA
0351 PLQKNQVVGT INFQLDGKTI EQRPLVVLQE IPEGNFFGKI IDYIKLMFHH WFG
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 acgttaacaa agtcacgcgg aaagtcagat
-070 gcctgccggt agtgcatttg ctatagtagg gcactttttt aattccatca cggatgtcgt agttcagacc
0001 atgaatacca ttttttccgc tcgtatcatg aagcgcctgg cgctcaccac ggctctttgc acagccttta
0071 tctctgctgc acatgccgat gacctgaata tcaaaactat gatcccgggt gtaccgcaga tcgatgcgga
0141 gtcctacatc ctgattgact ataactccgg caaagtgctc gccgaacaga acgcagatgt ccgccgcgat
0211 cctgccagcc tgaccaaaat gatgaccagt tacgttatcg gccaggcaat gaaagccggt aaatttaaag
0281 aaactgattt agtcactatc ggcaacgacg catgggccac cggtaacccg gtgtttaaag gttcttcgct
0351 gatgttcctc aaaccgggca tgcaggttcc ggtttctcag ctgatccgcg gtattaacct gcaatcgggt
0421 aacgatgctt gtgtcgccat ggccgatttt gccgctggta gccaggacgc ttttgttggc ttgatgaaca
0491 gctacgttaa cgcactgggc ctgaaaaata cccacttcca gacggtacat ggtctggatg ctgatggtca
0561 gtacagctcc gcgcgagata tggcgctgat cggccaggca ttgatccgtg acgtaccgaa tgaatactcg
0631 atctataaag aaaaagaatt tacgtttaac ggtattcgcc agctgaaccg taacggcctg ttatgggata
0701 acagcctgaa tgtcgacggc atcaaaaccg gacacactga caaagcaggt tacaaccttg ttgcttctgc
0771 gactgaaggc cagatgcgct tgatttctgc ggtaatgggc ggacgtactt ttaaaggccg tgaagccgaa
0841 agtaaaaaac tgctaacctg gggcttccgt ttctttgaaa ccgttaaccc actgaaagta ggtaaagagt
0911 tcgcctctga accggtttgg tttggtgatt ctgatcgcgc ttcgttaggg gttgataaag acgtgtacct
0981 gaccattccg cgtggtcgca tgaaagatct gaaagccagc tatgtgctga acagcagtga attgcatgcg
1051 ccgctgcaaa agaatcaggt cgtcggaact atcaacttcc agcttgatgg caaaacgatc gagcaacgcc
1121 cgctggttgt gttgcaagaa atcccggaag gtaacttctt cggcaaaatc attgattaca ttaaattaat
1191 gttccatcac tggtttggtt aaaaattaaa cacttgaaag tgtaatttcc gtccccatat actaagcatc
1261 agtaaaaaac tcccgccttc tggcgggagt tgctatttaa ttacgttacg cc
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