MG1655
W3110
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Gene Report : caiA
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCR28 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  caiA
Related strains
     Strains Search Search strains by gene name:  caiA   Search strains by all related name:  caiA b0039 ECK0040 JW0038 yaaO

General information  (Go to Linear View:)
 Gene Name caiA  
 Alternative name b0039,ECK0040,JW0038,yaaO  
 Location, Length 39,244 - 40,386 (  -  ) ;   0.85 min ; 1143 (bp) ,   380 (aa) Go to Linear View
 Product crotonobetaine reductase subunit II, FAD-binding  
 Operon Name caiTABCDE  
 Note probable carnitine operon oxidoreductase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006097 - glyoxylate cycle; GO_process: GO:0009310 - amine catabolic process; GO_process: GO:0042413 - carnitine catabolic process  
 Function putative regulator; Central intermediary metabolism: Pool, multipurpose conversions  
 Gene Ontology GO:0003995 ; acyl-CoA dehydrogenase activity ( caiA )
GO:0006118 ; electron transport ( caiA )
GO:0008152 ; metabolic process ( caiA )
GO:0016491 ; oxidoreductase activity ( caiA )
GO:0016627 ; oxidoreductase activity, acting on the CH-CH group of donors ( caiA )
GO:0050660 ; FAD binding ( caiA )
 PID 1786223  
 EC number
  (KEGG Pathway)   		1.3.99.-  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene caiA ( EG11560 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) CAIA_ECOLI ( P60584 ; P31571 )
    -  Protein name Crotonobetainyl-CoA dehydrogenase  
    -  Synonyms EC 1.3.99.-, Crotonobetainyl-CoA reductase  
    -  Gene name Name=caiA; OrderedLocusNames=b0039;  

 Linear View (Whole Mode)
View Location
   30.0  –  55.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP caiA (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Medium-Chain Acyl-Coa Dehydrogenase With 3-Thiaoctanoyl-Coa  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Crotonobetainyl-CoA dehydrogenase (Crotonobetainyl-CoA reductase)  
         PSI-BLAST Short-chain specific acyl-CoA dehydrogenase, mitochondrial precursor
    nr     (database updated : 2007.02.20 )
         BLAST crotonobetainyl-CoA dehydrogenase [Escherichia coli O157:H7 EDL933]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    ACYL_COA_DH_1    ACYL_COA_DH_2     

Other Cross-Reference
    COG COG1960I 
    EcoCyc caiA 

MMBR References
Mol Microbiol. 1994;13(5):775-86.
Molecular characterization of the cai operon necessary for carnitine metabolism in Escherichia coli.
Eichler K, Bourgis F, Buchet A, Kleber HP, Mandrand-Berthelot MA. ( 7815937 )
J Bacteriol. 1998;180:2599-2608
Regulation of the carnitine pathway in Escherichia coli: investigation of the cai-fix divergent promoter region.
Buchet, A., K. Eichler, and M. A. Mandrand-Berthelot.

Sequences
Amino acid
FASTA format 
0001 MDFNLNDEQE LFVAGIRELM ASENWEAYFA ECDRDSVYPE RFVKALADMG IDSLLIPEEH GGLDAGFVTL 
0071 AAVWMELGRL GAPTYVLYQL PGGFNTFLRE GTQEQIDKIM AFRGTGKQMW NSAITEPGAG SDVGSLKTTY 
0141 TRRNGKIYLN GSKCFITSSA YTPYIVVMAR DGASPDKPVY TEWFVDMSKP GIKVTKLEKL GLRMDSCCEI 
0211 TFDDVELDEK DMFGREGNGF NRVKEEFDHE RFLVALTNYG TAMCAFEDAA RYANQRVQFG EAIGRFQLIQ 
0281 EKFAHMAIKL NSMKNMLYEA AWKADNGTIT SGDAAMCKYF CANAAFEVVD SAMQVLGGVG IAGNHRISRF 
0351 WRDLRVDRVS GGSDEMQILT LGRAVLKQYR
Nucleotide
FASTA format

View sequence out neighbor 100bp 
-100                                             gctgttcttc gtcaacatta tggtgacgct 
-070 ctcctttatt aaagacgcga aacagaactg gaaagattaa ttaaccccca aaatatcaag aggttgaaag 
0001 atggatttta atttaaatga tgagcaggaa ctgtttgtcg ccggtatccg cgaactgatg gccagcgaaa 
0071 actgggaggc ctattttgcc gagtgcgacc gtgacagcgt ctacccggaa cgttttgtca aagcactggc 
0141 ggatatgggt atcgacagtc tgctgatccc tgaagagcac ggtggtctgg acgcggggtt tgttactctc 
0211 gccgccgtgt ggatggagct gggacgtctg ggggcaccaa cctatgtgct gtaccagttg ccgggcgggt 
0281 tcaacacctt cctgcgcgaa ggcacacaag agcagatcga caaaattatg gctttccgcg gcaccggtaa 
0351 gcagatgtgg aactcagcga ttaccgaacc gggcgcgggc tccgacgtgg gtagcctgaa aacgacttat 
0421 acccgtagaa atggtaagat ttatcttaat ggtagtaagt gttttattac cagcagcgcc tacaccccgt 
0491 acatcgtggt gatggcgcgc gacggggctt ctccggacaa acctgtctac accgaatggt ttgttgatat 
0561 gagcaaaccg ggcatcaaag tgaccaaact tgaaaagctc ggtctgcgta tggatagctg ctgtgaaatc 
0631 acctttgacg acgtggaact ggacgagaaa gacatgttcg gtcgggaagg taacggcttt aaccgcgtca 
0701 aagaagagtt cgaccatgaa cgtttcctgg tagccctcac caactacggt acggcgatgt gcgcctttga 
0771 agatgcggcg cgctacgcca atcagcgcgt gcagtttggc gaggctattg gtcgtttcca gttgattcag 
0841 gaaaaattcg cccacatggc gatcaaatta aactccatga aaaacatgct gtatgaagca gcgtggaaag 
0911 cagacaacgg caccatcacc tctggcgatg cagcgatgtg caaatacttc tgcgccaatg cggcatttga 
0981 agttgtggat agcgcaatgc aggtgctggg cggtgtcggg attgcgggca accaccgcat cagccgcttc 
1051 tggcgtgatc tgcgtgtaga ccgcgtctcc gggggatctg acgaaatgca gatcctgacg ctgggtcgtg 
1121 cggtgctgaa gcaataccgc taagtccgtg cgctgcctga tgcgacgctg atgcgtctta tcaggcctac 
1191 gggagcacga atgtaggccg gataaagcgt ttacgccgca tccggcagtc atg