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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
|
J Mol Biol. 1990;212(2):319-29.
Design and characterization of Escherichia coli mutants devoid of Ap4N-hydrolase activity.
Leveque F, Blanchin-Roland S, Fayat G, Plateau P, Blanquet S.
(
2157025
)
|
|
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Deletion
|
OCR29
(D)
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Related gene (W3110 PEC)
|
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Gene Search
|
Search MG1655 PEC by gene name:
apaH
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Related strains
|
|
Strains Search
|
Search strains by gene name:
apaH
Search strains by all related name:
apaH b0049 cfcB ECK0050 JW0048
|
General information (Go to Linear View: )
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|
Gene Name
|
apaH
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|
Alternative name
|
b0049,cfcB,ECK0050,JW0048
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|
Location, Length
|
50,380 - 51,222
(
-
)
;
1.09
min
;
843
(bp) ,
280
(aa)
|
|
Product
|
diadenosine tetraphosphatase
|
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Operon Name
|
lptD-surA-pdxA-rsmA-apaGH
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Note
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GO_component: GO:0005737 - cytoplasm; GO_process: GO:0015949 - nucleobase, nucleoside and nucleotide interconversion
|
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Function
|
enzyme; Salvage of nucleosides and nucleotides
|
|
Gene Ontology
|
GO:0008803
;
bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity ( apaH )
GO:0016787
;
hydrolase activity ( apaH )
|
|
PID
|
1786234
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EC number
(KEGG Pathway)
|
3.6.1.41
|
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SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
APAH_ECOLI
(
P05637
;
P78039
)
|
|
- Protein name
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Bis(5'-nucleosyl)-tetraphosphatase, symmetrical
|
|
- Synonyms
|
EC 3.6.1.41, Diadenosine tetraphosphatase, Ap4A hydrolase, Diadenosine 5',5'''- P1,P4-tetraphosphate pyrophosphohydrolase
|
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- Gene name
|
Name=apaH; OrderedLocusNames=b0049;
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Homology Analysis
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BLAST
|
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Bacteria
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GTOP
|
apaH
(
homologous genes of other bacterias
)
|
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PDB
(database updated :
2007.02.20
)
|
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PSI-BLAST
|
Chain A, Crystal Structure Of The Diadenosine Tetraphosphate Hydrolase From Shigella Flexneri 2a
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SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Bis
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|
PSI-BLAST
|
Bis
|
|
nr
(database updated :
2007.02.20
)
|
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BLAST
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diadenosinetetraphosphatase [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
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Pfam
|
|
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PROSITE
|
|
PROSITE
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PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
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MMBR References
|
Proc Natl Acad Sci U S A. 1989;86:5010-5014
An apaH mutation causes AppppA to accumulate and affects motility and catabolite repression in Escherichia coli.
Farr, S. B., D. N. Arnosti, M. J. Chamberlin, and B. N. Ames.
|
EMBO J. 1991;10:3897-3904
AppppA binds to several proteins in Escherichia coli, including the heat shock and oxidative stress proteins DnaK, GroEL, E89, C45, and C40.
Johnstone, D. B., and S. B. Farr.
|
J Mol Biol. 1990;212(2):319-29.
Design and characterization of Escherichia coli mutants devoid of Ap4N-hydrolase activity.
Leveque F, Blanchin-Roland S, Fayat G, Plateau P, Blanquet S.
(
2157025
)
|
J Bacteriol. 1985;164(1):63-9.
Molecular cloning of the Escherichia coli gene for diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase.
Mechulam Y, Fromant M, Mellot P, Plateau P, Blanchin-Roland S, Fayat G, Blanquet S.
(
2995325
)
|
J Bacteriol. 1989;171(9):4767-77.
Overlap between pdxA and ksgA in the complex pdxA-ksgA-apaG-apaH operon of Escherichia coli K-12.
Roa BB, Connolly DM, Winkler ME.
(
2670894
)
|
Mol Gen Genet. 1986;205(3):515-22.
The gene for Escherichia coli diadenosine tetraphosphatase is located immediately clockwise to folA and forms an operon with ksgA.
Blanchin-Roland S, Blanquet S, Schmitter JM, Fayat G.
(
3031429
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MATYLIGDVH GCYDELIALL HKVEFTPGKD TLWLTGDLVA RGPGSLDVLR YVKSLGDSVR LVLGNHDLHL
0071 LAVFAGISRN KPKDRLTPLL EAPDADELLN WLRRQPLLQI DEEKKLVMAH AGITPQWDLQ TAKECARDVE
0141 AVLSSDSYPF FLDAMYGDMP NNWSPELRGL GRLRFITNAF TRMRFCFPNG QLDMYSKESP EEAPAPLKPW
0211 FAIPGPVAEE YSIAFGHWAS LEGKGTPEGI YALDTGCCWG GTLTCLRWED KQYFVQPSNR HKDLGEAAAS
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 gcagggtcac tacgaaatga tcgatgaaaa
-070 tggcgtccct ttcagcatcg acattcccgt attccgactc gccgttccca cactcattca ttaaaagaat
0001 atggcgacat accttattgg cgacgttcat ggttgttacg atgaactgat cgcattgctg cataaagtag
0071 aatttacccc tgggaaagat accctctggc tgacgggcga tctggtcgcg cgcgggccgg gttcgctgga
0141 tgttctgcgc tatgtgaaat ccttaggcga cagcgtacgt ctggtgctgg gcaatcacga tctgcatctg
0211 ctggcggtat ttgccgggat cagccgcaat aaaccgaaag atcgcctgac accgctgctg gaagcgccgg
0281 atgccgacga gctgcttaac tggctgcggc gccagcctct gctgcaaatc gacgaagaga aaaagctggt
0351 gatggcccac gcagggatca cgccgcagtg ggatctgcag accgccaaag agtgcgcacg cgatgtagaa
0421 gcggtgctat cgagtgactc ctatcccttc tttcttgatg ccatgtacgg cgatatgcca aataactggt
0491 caccggaatt gcgggggctg ggaagactgc gttttatcac caacgctttt acccgtatgc gtttttgctt
0561 cccgaacggt caactggata tgtacagcaa agaatcgccg gaagaggccc ctgccccact gaaaccgtgg
0631 tttgcgattc ctggccctgt cgctgaagaa tacagcatcg cctttggtca ctgggcatcg ctggagggca
0701 aaggtacgcc ggaaggtata tacgcgctgg ataccggctg ctgctggggt ggtacattaa cctgcctgcg
0771 ctgggaagat aaacagtatt ttgtccagcc gtcgaaccgg cataaggatt tgggcgaagc ggcggcgtct
0841 taaacacagc ctgatatagg aaggccggat aagacgcgac cggcgtcgca tccggcgcta gccgtaaatt
0911 ctatacaaaa ttaccgccgc tccagaatct caa
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