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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
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DE(aroP-lpd)74
,
OCR31
(D)
,
OCR31-4
(D)
,
OCR31-7
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
aceF
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Related strains
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Strains Search
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Search strains by gene name:
aceF
Search strains by all related name:
aceF aceE2 b0115 ECK0114 JW0111
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General information (Go to Linear View: )
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Gene Name
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aceF
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Alternative name
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aceE2,b0115,ECK0114,JW0111
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Location, Length
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125,695 - 127,587
(
+
)
;
2.71
min
;
1893
(bp) ,
630
(aa)
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Product
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pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
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Operon Name
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pdhR-aceEF-lpd
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Note
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pyruvate dehydrogenase (dihydrolipoyltransacetylase component); GO_process: GO:0016052 - carbohydrate catabolic process; GO_process: GO:0006086 - acetyl-CoA biosynthetic process from pyruvate; GO_process: GO:0006096 - glycolysis; GO_process: GO:0009436 - glyoxylate catabolic process; GO_process: GO:0009061 - anaerobic respiration
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Function
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enzyme; Energy metabolism, carbon: Pyruvate dehydrogenase
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Gene Ontology
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GO:0004742
;
dihydrolipoyllysine-residue acetyltransferase activity ( aceF )
GO:0005515
;
protein binding ( aceF )
GO:0005737
;
cytoplasm ( aceF )
GO:0006096
;
glycolysis ( aceF )
GO:0008152
;
metabolic process ( aceF )
GO:0008415
;
acyltransferase activity ( aceF )
GO:0016740
;
transferase activity ( aceF )
GO:0031405
;
lipoic acid binding ( aceF )
GO:0045254
;
pyruvate dehydrogenase complex ( aceF )
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PID
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1786305
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EC number
(KEGG Pathway)
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2.3.1.12
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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aceF
(
EG10025
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
1
more]
)
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Entry name(Acc.no)
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ODP2_ECOLI
(
P06959
)
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- Protein name
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Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex
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- Synonyms
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EC 2.3.1.12, E2, Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex
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- Gene name
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Name=aceF; OrderedLocusNames=b0115;
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MMBR References
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Eur J Biochem. 1983;133:481-489
The pyruvate dehydrogenase complex of Escherichia coli K12. Nucleotide sequence encoding the dihydrolipoamide acetyltransferase component.
Guest, J. R., P. E. Stephens, M. G. Darlison, and H. M. Lewis.
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Biochem J. 1987;245(3):869-74.
Subgenes expressing single lipoyl domains of the pyruvate dehydrogenase complex of Escherichia coli.
Miles JS, Guest JR.
(
3117051
)
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FEMS Microbiol Lett. 1998;159(2):325-9.
Environmental control of pyruvate dehydrogenase complex expression in Escherichia coli.
Cassey B, Guest JR, Attwood MM.
(
9503628
)
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| Sequences |
Amino acid
FASTA format
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0001 MAIEIKVPDI GADEVEITEI LVKVGDKVEA EQSLITVEGD KASMEVPSPQ AGIVKEIKVS VGDKTQTGAL
0071 IMIFDSADGA ADAAPAQAEE KKEAAPAAAP AAAAAKDVNV PDIGSDEVEV TEILVKVGDK VEAEQSLITV
0141 EGDKASMEVP APFAGTVKEI KVNVGDKVST GSLIMVFEVA GEAGAAAPAA KQEAAPAAAP APAAGVKEVN
0211 VPDIGGDEVE VTEVMVKVGD KVAAEQSLIT VEGDKASMEV PAPFAGVVKE LKVNVGDKVK TGSLIMIFEV
0281 EGAAPAAAPA KQEAAAPAPA AKAEAPAAAP AAKAEGKSEF AENDAYVHAT PLIRRLAREF GVNLAKVKGT
0351 GRKGRILRED VQAYVKEAIK RAEAAPAATG GGIPGMLPWP KVDFSKFGEI EEVELGRIQK ISGANLSRNW
0421 VMIPHVTHFD KTDITELEAF RKQQNEEAAK RKLDVKITPV VFIMKAVAAA LEQMPRFNSS LSEDGQRLTL
0491 KKYINIGVAV DTPNGLVVPV FKDVNKKGII ELSRELMTIS KKARDGKLTA GEMQGGCFTI SSIGGLGTTH
0561 FAPIVNAPEV AILGVSKSAM EPVWNGKEFV PRLMLPISLS FDHRVIDGAD GARFITIINN TLSDIRRLVM
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 GTGGCGAAAT CGATAAGAAA GTGGTTGCTG
-070 ACGCAATCGC CAAATTCAAC ATCGATGCAG ATAAAGTTAA CCCGCGTCTG GCGTAAGAGG TAAAAGAATA
0001 atggctatcg aaatcaaagt accggacatc ggggctgatg aagttgaaat caccgagatc ctggtcaaag
0071 tgggcgacaa agttgaagcc gaacagtcgc tgatcaccgt agaaggcgac aaagcctcta tggaagttcc
0141 gtctccgcag gcgggtatcg ttaaagagat caaagtctct gttggcgata aaacccagac cggcgcactg
0211 attatgattt tcgattccgc cgacggtgca gcagacgctg cacctgctca ggcagaagag aagaaagaag
0281 cagctccggc agcagcacca gcggctgcgg cggcaaaaga cgttaacgtt ccggatatcg gcagcgacga
0351 agttgaagtg accgaaatcc tggtgaaagt tggcgataaa gttgaagctg aacagtcgct gatcaccgta
0421 gaaggcgaca aggcttctat ggaagttccg gctccgtttg ctggcaccgt gaaagagatc aaagtgaacg
0491 tgggtgacaa agtgtctacc ggctcgctga ttatggtctt cgaagtcgcg ggtgaagcag gcgcggcagc
0561 tccggccgct aaacaggaag cagctccggc agcggcccct gcaccagcgg ctggcgtgaa agaagttaac
0631 gttccggata tcggcggtga cgaagttgaa gtgactgaag tgatggtgaa agtgggcgac aaagttgccg
0701 ctgaacagtc actgatcacc gtagaaggcg acaaagcttc tatggaagtt ccggcgccgt ttgcaggcgt
0771 cgtgaaggaa ctgaaagtca acgttggcga taaagtgaaa actggctcgc tgattatgat cttcgaagtt
0841 gaaggcgcag cgcctgcggc agctcctgcg aaacaggaag cggcagcgcc ggcaccggca gcaaaagctg
0911 aagccccggc agcagcacca gctgcgaaag cggaaggcaa atctgaattt gctgaaaacg acgcttatgt
0981 tcacgcgact ccgctgatcc gccgtctggc acgcgagttt ggtgttaacc ttgcgaaagt gaagggcact
1051 ggccgtaaag gtcgtatcct gcgcgaagac gttcaggctt acgtgaaaga agctatcaaa cgtgcagaag
1121 cagctccggc agcgactggc ggtggtatcc ctggcatgct gccgtggccg aaggtggact tcagcaagtt
1191 tggtgaaatc gaagaagtgg aactgggccg catccagaaa atctctggtg cgaacctgag ccgtaactgg
1261 gtaatgatcc cgcatgttac tcacttcgac aaaaccgata tcaccgagtt ggaagcgttc cgtaaacagc
1331 agaacgaaga agcggcgaaa cgtaagctgg atgtgaagat caccccggtt gtcttcatca tgaaagccgt
1401 tgctgcagct cttgagcaga tgcctcgctt caatagttcg ctgtcggaag acggtcagcg tctgaccctg
1471 aagaaataca tcaacatcgg tgtggcggtg gataccccga acggtctggt tgttccggta ttcaaagacg
1541 tcaacaagaa aggcatcatc gagctgtctc gcgagctgat gactatttct aagaaagcgc gtgacggtaa
1611 gctgactgcg ggcgaaatgc agggcggttg cttcaccatc tccagcatcg gcggcctggg tactacccac
1681 ttcgcgccga ttgtgaacgc gccggaagtg gctatcctcg gcgtttccaa gtccgcgatg gagccggtgt
1751 ggaatggtaa agagttcgtg ccgcgtctga tgctgccgat ttctctctcc ttcgaccacc gcgtgatcga
1821 cggtgctgat ggtgcccgtt tcattaccat cattaacaac acgctgtctg acattcgccg tctggtgatg
1891 taaGTAAAAG AGCCGGCCCA ACGGCCGGCT TTTTTCTGGT AATCTCATGA ATGTATTGAG GTTATTAGCG
1961 AATAGACAAA TCGGTTGCCG TTTGTTGTTT AAA
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