How to Deposit a Strain

• 1. Select a disrupted gene.
• 2. Read the terms and conditions.
• 3. Sign up or login.
• 4. Enter deposit strain information.
• 5. Confirm the depositor information and deposit strain information.
• 6. This completes your request for strain deposit.

1. Select a disrupted gene.
*Click figure to enlarge.
	1.1	Click on "Strain deposit" in the menu to view the disrupted gene list.
	1.2	Select a disrupted gene in the strain you wish to deposit. Click on a letter to view the list of disrupted genes beginning with the selected letter. Click on to select the appropriate disrupted gene. Click on if you wish to cancel.
	1.3	You can select multiple disrupted genes. When you have finished selecting all the genes, click on the button.
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2. Read the terms and conditions.
*Click figure to enlarge.	2.1	Read the terms and conditions for strain deposit. If you agree to them, check "I agree" and click .
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3. Sign up or login.
	3.1	If you have not signed up as a user, click on the button. You will proceed to the depositor information page as shown in step 3.2. If you have signed up with Bacillus subtilis, enter your email address and click on the button. You will proceed to the deposit strain information page as shown in step 4.
*Click figure to enlarge.
	3.2	First time depositors should fill in their personal information and click "Next". Please do not use half-width Katakana or machine dependent characters because it may cause some text to become garbled. Required fields are marked with an asterisk *.
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4. Enter deposit strain information.
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	4.1	Enter the deposit strain information. If you click on "Add deposit strain", a new deposit strain form will be added to the bottom of the page. If you click on "Remove deposit strain", the deposit strain form will be removed. (If there is only 1 remaining deposit strain, you will not be able to remove it. Instead, the deposit strain form will be cleared. )
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5. Confirm the depositor information and deposit strain information.
*Click figure to enlarge.
	5.1	Confirm the depositor information.  Click on "Change depositor info" if you wish to change the depositor’s information.
	5.2	Confirm the deposit strain information.  Click on "Change deposit strain info" if you wish to change the deposit strain information.
	5.3	When you are ready to submit your request, click on the "Send" button.
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6. This completes your request for strain deposit.
*Click figure to enlarge.	6.1	This completes your request for strain deposit. A confirmation email will be sent to the depositor's email address.
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