Strain - 詳細 -
分譲依頼
|
|
株番号
|
NIES-88
|
保存機関名
|
国立環境研究所
|
門名
|
Cyanophyta
|
綱名
|
Cyanophyceae
|
目名
|
|
科名
|
|
学名
|
Microcystis aeruginosa (Kützing) Lemmermann
|
シノニム
|
Microcystis ichthyoblabe Kützing; Microcystis novacekii (Komárek) Compère; Microcystis viridis (A.Brown) Lemmermann; Microcystis wesenbergii Komárek
|
一般名
|
藍藻; シアノバクテリア
|
地名(採集年月)
|
日本 山梨県
河口湖
(1981-06-**)
|
採集者名
|
|
生息環境(分離源)
|
淡水
|
生息環境の詳細
|
富栄養 湖沼
|
分離者名(分離年月)
|
渡邉途子
|
同定者名
|
渡辺途子
|
株の状態
|
非無菌; 単藻; クローン
|
保存形態
|
凍結保存
|
凍結保存
|
可
|
培養条件(前培養条件)
|
培地名:MA
培地形状:液体
温度:25
℃
光強度:20-30
μmol phtons/m2/sec
明暗周期:10L:14D
継代培養周期:20 D
|
遺伝子情報
|
16S rRNA
(AB023255
)
ftsZ
(AB324852
)
glnA
(AB324931
)
gltX
(AB325010
)
gyrB
(AB325089
)
mcyA
(AB110103
)
mcyD
(AB110114
)
mcyG
(AB110125
)
mcyJ
(AB110136
)
pgi
(AB325168
)
recA
(AB325247
)
tpi
(AB325326
)
Whole-genome
(JXYX00000000
)
|
特性
|
アオコ; 生物指標; 有毒; ミクロシスチン(+) (Tanabe et al. 2009b)
ST3 (Tanabe et al. 2007); ゲノム解読株 (Parajuli et al. 2016)
|
細胞サイズ(min-max)
|
5μm
|
体制
|
|
注意事項
|
凍結保存; 有毒
|
|
文献
|
Ishida, K., Matsuda, H., Murakami, M.:
1998:
Micropeptins 88-A to 88-F, chymotrypsin inhibitors from the cyanobacterium Microcystis aeruginosa (NIES-88).:
Tetrahedron,
54,
5545-5556.
Strains:
88
Ishida, K., Matsuda, H., Murakami, M., Yamaguchi, K.:
1996:
Kawaguchipeptin A, a novel cyclic undecapeptide from cyanobacterium Microcystis aeruginosa (NIES-88).:
Tetrahedron,
52,
9025-9030.
Strains:
88
Ishida, K., Matsuda, H., Murakami, M., Yamaguchi, K.:
1997:
Kawaguchipeptin B, an antibacterial cyclic undecapeptide from the cyanobacterium Microcystis aeruginosa.:
J. Nat. Prod.,
60,
724-726.
Strains:
88
Jang, M-H., Ha, K., Joo, G-J., Takamura, N.:
2003:
Toxin production of cyanobacteria is increased by exposure to zooplankton.:
Freshw. Biol.,
48,
1540-1550.
Strains:
44
87
88
99
Kaya, K. & Sano, T.:
1998:
A photodetoxifi-cation mechanism of the cyanobacterial hepatotoxin microcystin-LR by ultraviolet irradiation.:
Chem. Res. Toxicol.,
11,
159-163.
Strains:
88
Kondo, R., Kagiya, G., Hiroishi, S., Watanabe, M.:
2000:
Genetic typing of a bloom-forming cyanobacterial genus Microcystis in Japan using 16S rRNA gene sequence analysis.:
Plankton Biol. Ecol.,
47,
1-6.
Strains:
87
88
89
90
91
98
99
100
101
102
104
105
106
108
109
110
111
112
298
299
604
1055
1061
1068
1076
1090
1091
1105
1122
1142
1164
Kondo, R., Kagiya, G., Hiroishi, S., Watanabe, M., Hata, Y.:
1999:
Rapid DNA extraction from a bloom-forming cyanobacterium.:
Microbes Environ.,
14,
157-161.
Strains:
87
88
89
90
91
98
99
100
101
102
104
105
106
108
109
110
111
112
298
299
604
1055
1061
1068
1076
1090
1091
1105
1122
1142
1164
Kondo, R., Kagiya, G., Komura, M., Yuki, Y., Hiroishi, S., Watanabe, M.:
2000:
Molecular taxonomy of a bloom-forming cyanobacterial genus Microcystis.:
Bull. Plankton Soc. Japan,
47,
25-33 (in Japanese with English summary).
Strains:
6
87
88
89
90
91
98
99
100
101
102
104
105
106
108
109
110
111
112
298
299
604
1055
1061
1068
1076
1090
1091
1105
1122
1142
1164
Kondo, R., Kagiya, G., Yuki, Y., Hiroishi, S., Watanabe, M.:
1998:
Taxonomy of a bloom-forming cyanobacterial genus Microcystis.:
Nippon Suisan Gakkaishi,
64,
291-292 (in Japanese with English title).
Strains:
87
88
89
90
91
98
99
100
101
102
104
105
106
107
108
109
110
111
112
298
299
604
Soma, Y., Imaizumi, T., Yagi, K., Kasuga, S.:
1993:
Estimation of algal succession in lake water using HPLC analysis of pigments.:
Can. J. Fish. Aquat. Sci.,
50,
1142-1146.
Strains:
44
73
81
88
104
111
233
234
333
351
Tsuchiya, Y., Watanabe, M. F., Watanabe, M.:
1992:
Volatile organic sulfur compounds associated with blue-green algae from inland waters of Japan.:
Wat. Sci. Tech.,
25,
123-130.
Strains:
88
1061
1080
1081
1083
1098
1104
1137
Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T.:
1984:
Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing.:
Jpn. J. Freez. Dry.,
30,
23-26 (in Japanese with English title).
Strains:
18
19
21
24
26
29
31
39
40
41
44
45
46
50
73
74
75
76
77
80
81
87
88
89
90
91
93
95
98
99
100
101
120
122
131
135
137
144
151
204
207
213
257
263
379
415
2219
2235
2239
2579
Yoshida, T., Yuki, Y., Lei, S., Chinen, H., Yoshida, M., Kondo, R., Hiroishi, S.:
2003:
Quantitative detection of toxic strains of the cyanobacterial genus Microcystis by competitive PCR.:
Microbes Environ.,
18,
16-23.
Strains:
87
88
89
90
102
104
105
106
108
109
110
111
112
298
299
604
Yuki, Y., Yoshida, T., Hiroishi, S.:
2002:
Molecular detection of the toxic cyanobacteria [Yûdoku aoko no bunshi-shikibetsu to yosatsu he-no ôyô].:
In Series of Fisheries Science Vol. 143, Prevention and Extermination Strategies of Harmful Algal Blooms [Suisan-gaku shirîzu 134, Yûgai-yûdoku sôrui burûmu no yobô to kujo] , Eds. by Hiroishi, S., Imai, I. & Ishimaru, T., Koseisha-Koseikaku, Tokyo,
pp. 43-53 (in Japanese).
Strains:
73
81
87
88
89
90
91
98
99
100
101
102
104
105
106
108
109
110
111
112
204
230
298
299
604
Jang, M. H., Ha, K., Lucas, M. C., Joo, G. J., Takamura, N.:
2004:
Changes in microcystin production by Microcystis aeruginosa exposed to phytoplanktivorous and omnivorous fish.:
Aquat. Toxicol.,
68,
51-59.
Strains:
44
88
99
Yamaki, H., Sitachitta, N., Sano, T., Kaya, K.:
2005:
Two new chymotrypsin inhibitors isolated from the cyanobacterium Microcystis aeruginosa NIES-88.:
J. Nat. Prod.,
68,
14-18.
Strains:
88
Tanabe, Y., Kaya, K., Watanabe, M. M.:
2004:
Evidence for recombination in the microcystin synthetase (mcy) genes of toxic cyanobacteria Microcystis spp.:
J. Mol. Evol.,
58,
633-641.
Strains:
88
89
90
102
103
107
Tsuda, K., Takamura, N., Matsuyama, M., Fujii, Y.:
2005:
Assessment method for leaf litters allelopathic effect on cyanobacteria.:
J. Aquat. Plant Manage,
43,
43-46.
Strains:
88
Jang, M.-H., Ha, K., Jung, J.-M., Lee, Y.-J., Takamura, N.:
2006:
Increased microcystin production of Microcystis aeruginosa by indirect exposure of nontoxic cyanobacteria: potential role in the development Microcystis bloom.:
Bull. Environ. Contam. Toxicol.,
76,
957-962.
Strains:
74
88
204
Jang, M.-H., Jung, J.-M., Takamura, N.:
2007:
Changes in microcystin production in cyanobacteria exposed to zooplankton at different population densities and infochemical concentrations.:
Limnol. Oceanogr.,
52,
1454-1466.
Strains:
88
98
107
Jang, M.-H., Ha, K., Takamura, N.:
2008:
Microcystin production by Microcystis aeruginosa exposed to different stages of herbivorous zooplankton.:
Toxicon,
51,
882-889.
Strains:
88
98
103
107
Tanabe, Y., Kasai, F., Watanabe, M. M.:
2007:
Multilocus sequence typing (MLST) reveals high genetic diversity and clonal population structure of the toxic cyanobacterium Microcystis aeruginosa.:
Microbiology,
153,
3695-3703.
Strains:
44
87
88
89
90
91
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
298
299
478
604
843
901
933
1050
1051
1052
1053
1054
1055
1056
1057
1058
1059
1060
1061
1062
1063
1064
1065
1066
1067
1068
1069
1070
1072
1075
1076
1077
1085
1090
1091
1092
1093
1094
1095
1096
1097
1098
1100
1101
1102
1103
1104
1105
1106
1107
1108
1113
1114
1115
1122
1123
1124
1125
1126
1127
1128
1129
1130
1131
1132
1133
1134
1135
1136
1137
1138
1139
1140
1141
1142
1143
1144
1145
1146
1147
1148
1149
1150
1151
1152
1153
1155
1156
1157
1159
1160
1161
1162
1163
1164
1170
1171
1172
1209
1211
1213
1214
1215
1216
1217
1218
1219
1220
1221
1222
1223
1224
1225
1226
1227
1228
1229
1230
1231
1232
1233
1234
1235
1236
1237
1238
1239
1240
1241
1242
1243
1244
1245
1251
1252
1253
1254
Tanabe, Y., Sano, T., Kasai, F., Watanabe, M. M.:
2009b:
Recombination, cryptic clades and neutral molecular divergence of the microcystin synthetase (mcy) genes of toxic cyanobacterium Microcystis aeruginosa. :
BMC Evol. Biol.,
9,
115 (article ID).
Strains:
44
87
88
89
90
91
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
298
299
478
604
843
901
1050
1051
1052
1053
1054
1055
1056
1057
1058
1059
1060
1061
1062
1063
1064
1065
1066
1067
1068
1069
1070
1072
1075
1076
1077
1085
1090
1091
1092
1093
1094
1095
1096
1097
1098
1100
1101
1102
1103
1104
1105
1106
1107
1108
1113
1114
1115
1122
1123
1124
1125
1126
1127
1128
1129
1130
1131
1132
1133
1134
1135
1136
1137
1138
1139
1140
1141
1142
1143
1144
1145
1146
1147
1148
1149
1150
1151
1152
1153
1155
1156
1157
1159
1160
1161
1162
1163
1164
1170
1171
1172
1209
1211
1213
1214
1215
1216
1217
1218
1219
1220
1221
1222
1223
1224
1225
1226
1227
1228
1229
1230
1231
1232
1233
1234
1235
1236
1237
1238
1239
1240
1241
1242
1243
1244
1245
1251
1252
1253
1254
2465
2466
2467
2469
2470
2471
2472
2473
2474
2475
2476
2477
2478
2479
2561
Kimura, J. :
2011:
Screening for allelopathic activity on algal growth. :
Bull. Hiroshima Pref. Tech. Res. Inst., Publ. Health Environ. Center,
19,
37-46 (in Japanese).
Strains:
88
487
642
Tanabe, Y. & Watanabe, M. M. :
2011:
Local expansion of a panmictic lineage of water bloom-forming cyanobacterium Microcystis aeruginosa. :
PLoS One,
6,
e17085 (article ID).
Strains:
44
87
88
89
90
91
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
298
299
478
604
843
901
1050
1051
1052
1053
1054
1055
1056
1057
1058
1059
1060
1061
1062
1063
1064
1065
1066
1067
1068
1069
1070
1072
1075
1076
1077
1085
1090
1091
1092
1093
1094
1095
1096
1097
1098
1100
1101
1102
1103
1104
1105
1106
1107
1108
1113
1114
1115
1122
1123
1124
1125
1126
1127
1128
1129
1130
1131
1132
1133
1134
1135
1136
1137
1138
1139
1140
1141
1142
1143
1144
1145
1146
1147
1148
1149
1150
1151
1152
1153
1155
1156
1157
1159
1160
1161
1162
1163
1164
1170
1171
1172
1173
1174
1175
1176
1177
1179
1180
1181
1183
1184
1185
1186
1209
1211
1213
1214
1215
1216
1217
1218
1219
1220
1221
1222
1223
1224
1225
1226
1227
1228
1229
1230
1231
1232
1233
1234
1235
1236
1237
1238
1239
1240
1241
1242
1243
1244
1245
1251
1252
1253
1254
2088
2089
2090
2091
2465
2466
2467
2468
2469
2470
2471
2472
2473
2474
2475
2476
2477
2478
2479
2480
2481
2482
2483
2484
2485
2486
2487
2488
2489
2490
2491
2492
2493
2494
2495
2496
2542
2543
2544
2545
2546
2547
2548
2549
2550
2551
2552
2553
2554
2555
2556
2557
2558
2559
2560
2561
2591
2592
2593
2594
2595
2596
2597
2598
2599
2600
2601
2602
2603
2604
2605
2606
2607
2608
2609
2610
2611
2612
2613
2617
2618
2619
2620
2621
2622
2623
2624
2625
2626
2627
2628
2629
Kubo, T., Kato, N., Hosoya, K., Kaya, K.:
2008:
Effective determination method for a cyanobacterial neurotoxin, β-N-methylamino-L-alanine.:
Toxicon,
51,
1264-1268.
Strains:
88
Kimura, J.:
2011:
Screening for allelopathic activity on algal growth.:
Bull. Hiroshima Pref. Tech. Res. Inst., Publ. Health Environ. Center,
19,
37-46 (in Japanese with English title).
Strains:
88
487
642
Sun, L.W., Jiang, W.J., Sato, H., Kawachi, M., Lu, X.W.:
2016:
Rapid classification and identification of Microcystis aeruginosa strains using MALDI-TOF MS and polygenetic analysis.:
PLoS One,
11,
e0156275 (article ID).
Strains:
44
87
88
89
90
100
101
104
105
107
109
111
112
478
604
843
901
1043
1053
1058
1061
1068
1069
1070
1072
1076
1077
1085
1094
1099
1101
1104
1105
1113
1115
1126
1134
1135
1144
1151
1157
1170
1211
1213
1215
1216
1217
1218
1222
1224
1230
1232
1233
1239
Parajuli, A., Kwak, D. H., Dalponte, L., Leikoski, N., Galica, T., Umeobika, U., Trembleau, L., Bent, A., Sivonen, K., Wahlsten, M.:
2016:
A unique tryptophan c-prenyltransferase from the kawaguchipeptin biosynthetic pathway.:
Angew. Chem.-Int. Edit.,
7,
3596-3599.
Strains:
88
Tanabe, Y., Hodoki, Y., Sano, T., Tada, K., Watanabe, MM.:
2018a:
Adaptation of the freshwater bloom-forming cyanobacterium Microcystis aeruginosa to brackish water is driven by recent horizontal transfer of sucrose genes.:
Front. Microbiol.,
9,
1150 (article ID).
Strains:
44
87
88
89
90
91
98
99
100
101
102
103
104
105
107
108
109
112
298
299
478
604
843
901
1050
1052
1059
1063
1067
1069
1070
1075
1076
1085
1093
1094
1098
1100
1101
1102
1104
1105
1106
1107
1108
1113
1114
1115
1122
1128
1130
1131
1132
1134
1139
1141
1142
1143
1144
1150
1170
1174
1175
1176
1177
1179
1181
1183
1185
1211
1213
1214
1215
1232
1234
1238
1239
1252
2088
2090
2091
2465
2466
2467
2468
2469
2470
2471
2472
2473
2475
2476
2477
2478
2479
2480
2481
2482
2483
2484
2485
2486
2487
2488
2489
2490
2491
2492
2494
2495
2546
2547
2548
2549
2550
2551
2552
2553
2554
2555
2556
2557
2558
2559
2560
2561
2592
2594
2595
2596
2597
2598
2599
2600
2601
2602
2603
2604
2605
2606
2607
2608
2610
2611
2612
2613
2617
2618
2619
2620
2621
2622
2623
2624
2625
2626
2628
2629
4234
Tsuchiya, R., Kihira, M., Sakanoue, Y., Araki, T.:
2018:
Assesment of inhibition effect on growth of Microcystis aeruginosa by autoclaved water extracts from leaves of 104 woody plant species.:
Jpn. J. Limnol.,
79,
41-48 (in Japanese with English summary).
Strains:
88
Chen, M., Tian, L.-L., Ren, C.-Y., Xu, C.-Y., Wang, Y.-Y., Li, L.:
2019:
Extracellular polysaccharide synthesis in a bloom-forming strain of Microcystis aeruginosa: implications for colonization and buoyancy.:
Sci Rep,
9,
1251 (article ID).
Strains:
87
88
98
204
298
843
2481
2549
MacCready, J. S., Basalla, J. L., Vecchiarelli, A. G.:
2020:
Origin and evolution of carboxysome positioning systems in cyanobacteria.:
Mol. Biol. Evol.,
37,
1434–1451.
Strains:
21
22
25
26
30
39
44
73
87
88
267
592
806
843
932
970
2098
2100
2107
2108
2111
2549
3275
3754
3756
3974
4073
4074
4075
4101
4103
4106
Erratt, K. J., Creed, I. F., Trick, C. G.:
2020:
Differential drawdown of ammonium, nitrate, and urea by freshwater chlorophytes and Cyanobacteria.:
J. Phycol.,
56,
458-468.
Strains:
88
90
102
843
Pound, H. L. & Wilhelm, S. W.:
2020:
Tracing the active genetic diversity of Microcystis and Microcystis phage through a temporal survey of Taihu.:
PLoS One,
15,
e0244482 (article ID).
Strains:
88
843
Phan, C.-S., Matsuda, K., Balloo, N., Fujita, K., Wakimoto, T., Okino, T.:
2021:
Argicyclamides A–C Unveil Enzymatic Basis for Guanidine Bis-prenylation:
J. Am. Chem. Soc.,
143,
10083–10087.
Strains:
88
Cao, H., Xu, D., Zhang, T., Ren, Q., Xiang, L., Ning, C., Zhang, Y., Gao, R.:
2022:
Comprehensive and functional analyses reveal the genomic diversity and potential toxicity of Microcystis.:
Harmful Algae,
113,
102186 (article ID).
Strains:
88
102
298
843
2481
2549
Dahedl, E. K. & Urakawa, H.:
2023:
Differential effects of hydrogen peroxide and L-lysine treatments on the growth of freshwater Cyanophyta and Chlorophyta.:
Ecologies,
4,
355-370.
Strains:
88
102
843
4325
Balloo, N., Mehjabin, J. J., Phan, C.-S., Okino, T.:
2023:
Heat shock and iron limitation modulate the metabolic profile of the cyanobacterium Microcystis aeruginosa NIES-88.:
Phycol. Res.,
71,
200-208.
Strains:
88
Zzzbe:
2022:
Chapter 2 - Specialized metabolites from cyanobacteria and their biological activities.:
In The Pharmacological Potential of Cyanobacteria, Eds. by Lopes, G., Silva, M., Vasconcelos, V., Academic press
pp. 21-54.
Strains:
88
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